Banca de QUALIFICAÇÃO: DIMITRY MACEDONIO FRACARO BALDISSERA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : DIMITRY MACEDONIO FRACARO BALDISSERA
DATA : 13/12/2019
HORA: 15:30
LOCAL: IFC Campus Concórdia
TÍTULO:

CITRATE / CREATININE RATIO REFERENCE RANGE IN ISOLATED URIN SAMPLES OF HEALTHY DOGS

PALAVRAS-CHAVES:

Hypocitraturia, urinalysis, urolithiasis, crystallization inhibitors, citric acid

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

The citrate / creatinine ratio serves as a marker of hypocitraturia in the diagnostic aid of urolithiasis in humans. The urolithiasis is the third most important disease of the domestic canine urinary system, factors such as the decrease in the concentration of urinary crystallization inhibitors, progressively increase the risk of formation of uroliths, being of great relevance in the diagnostic aid, the use of laboratory tests to evaluate cases of urolithiasis. The urinary citrate in human medicine acts as an inhibitor of calcium oxalate stone formation, whereas the low urinary citrate excretion (hypocitraturia) is a well-accepted risk factor for urolith formation. The purpose of this study was to determine the reference range of the citrate / creatinine ratio in random urine samples in healthy dogs. To date, there have been 34 isolated urine specimens collected by cystocentesis or spontaneous urination from dogs attended at the IFC-Campus Concordia Center for Clinical and Surgical Practice and at the private veterinary clinic “Le-Petit”, both in Concórdia-SC. For this, the animals were divided into groups according to gender, Group I (males n = 12), Group II (females n = 22) and by age group, Group III (adults n = 19) and Group IV (senile n = 15). After collect, the samples were refrigerated at 8ºC, whereas each sample was transported within 72 hours after collect to the “Analic Laboratory”, located in Concórdia, where they were immediately submitted to physicochemical analysis and urinary sediment, automated, to rule out the presence of proteinuria, as well as the presence of hematuria, leucocyturia, bacteriuria, crystalluria and cylindruria, samples that presented some of these abnormalities were discarded from the study. Then, the samples were transported refrigerated, acidified with 50% hydrochloric acid and sent to the Hermes Pardini Institute in Belo Horizonte-MG, where the quantifications of citrate, creatinine levels were analyzed and the citrate / creatinine ratio was calculated by the veterinary sector. The data were submitted to statistical analysis to calculate the average, standard deviation, median and coefficient of variation of the analyzed variables, being used the Kruskal Wallis test for nonparametric variables. The twelve dogs included in Group I had an average age of 8,25±2,98 years old and median for urinary citrate levels at 5.2 mg / dL, urinary creatinine levels at 56.45 mg / dL and citrate / creatinine ratio of 0.15, Group II: 9,81±4,79, 10,9, 93,0 and 0,12, Group III: 6,21±2,48, 6,6, 58,5 and 0,11 and Group IV: 13,13±2,50, 11,7, 82,3 and 0,13, respectively. The data show that male dogs had the lowest levels of urinary citrate and creatinine, when compared to females, although the difference was not statistically significant. The same occurs when comparing adult dogs with senile dogs, which corroborates studies that point to a higher incidence of urolithiasis in male dogs, as well as low urinary citrate levels in male humans. The values of citrate / creatinine ratios also did not show statistically significant differences when compared between groups, indicating a single reference range for dogs, similar to what occurs in human medicine.

MEMBROS DA BANCA:
Presidente - 1081425 - TEANE MILAGRES AUGUSTO GOMES
Interno - 2017813 - RICARDO EVANDRO MENDES
Interna - 1989957 - ALESSANDRA FARIAS MILLEZI