Banca de QUALIFICAÇÃO: ALESSANDRA APPEL
Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.DISCENTE : ALESSANDRA APPEL
DATA : 13/12/2019
HORA: 15:20
LOCAL: IFC- Campus Araquari
TÍTULO:
SHAVING DISINFECTION, FOR POULTRY USE, WITH PHOTOHYDROIONIZATION AND ULTRAVIOLET.
PALAVRAS-CHAVES:
PHI and UV, Poultry, Biosecurity, Sanitize
PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:
INTRODUCTION - Biosecurity is a determining factor for poultry activity, established through good practices to mitigate the challenges in the production of animals against pathogens. The procedures implemented in the farms, such as the intensive care of cleaning and disinfection of the facilities and all materials that will have contact with the animals are essential for disease prevention. In this regard, alternatives are constantly applied for disinfection in the production process and the involved molds, such as the case of shavings, used in the poultry litter and nests. The present work aims to test the disinfection of shavings through photohydroionization and ultraviolet lamp. OBJECTIVE - To evaluate the disinfection efficiency of photohydroionization and ultraviolet lamp on new shavings, used as substrate for litter and nests in commercial poultry. METHODS - The experiment was carried out with 15 kg of new shaving sterilized by autoclaving and challenged with pre-established bacterial, fungal and viral inoculum. The bacterial agents selected were Escherichia coli strains, Staphylococcus aureus and Salmonella Abony. The fungal agent was Saccharomyces cerevisiae and the viral agent used was Gumboro virus, wild strain. 250 grams of shavings were used for each batch, challenged with 32 ml of bacterial and fungal inoculum and 10 ml of viral inoculum, and 6 repetitions were applied at four different times: 0, 1, 5 and 10 minutes. The disinfection process was performed in a benchtop photohydroionizer equipped with 4 ultraviolet lamps. The shaving was inoculated with the agents distinctly and submitted to the disinfection system at the time. The inoculated agents in the shaving were recovered after the disinfection process as follows: Bacteriological analysis: CFU / g Count of Total Bacteria and Total Enterobacteria, respectively on MacConkey Agar and PCA (Plate Count Agar). Fungal analysis: Shavings were inoculated into PDA (Potato Dextrose Agar) selective culture, and the remaining colonies were counted per litter gram (CFU / g). Virological analysis: Samples were inoculated into embryonated SPF (Specific Pathogen Free) eggs for viral detection by reverse transcription polymerase (RT-qPCR). Data were analyzed in a completely randomized design and submitted to descriptive statistics analysis and the Shapiro Wilk normality test. To evaluate the effects of photohydroionization and ultraviolet time on inactivation of bacteria, viruses and fungi the data were submitted to regression analysis (REG) and broken line regression (NLIN) to describe changes in the concentration of bacteria, viruses and fungi with increasing photohydroionization and ultraviolet time. RESULTS - Mean values of 4.0 were observed; 2.56; 1.70 and 1.77 CFU / g of total bacteria, 4.0; 0.90; 0.50 and 0.33 CFU / g of total entorobacteria and 4.00; 2.64; 2.51 and 2.38 CFU / g of fungi at times 0, 1, 5 and 10 minutes, respectively. Total bacterial and enterobacterial as well as fungal counts showed quadratic effect with increased time of photohydroionization and ultraviolet process (Y = 3.36957 - 0.55224x + 0.03953x2; Y = 2.288775 - 0.64015x + Y = 3.24030 - 0.25772x + 0.01747x2, respectively). The Linear Response Plateau - (LRP) effect Total Bacteria variable (P <0.0001) was observed, with a minimum time point of 1.57 (R) minutes at minimum contamination of 1.74 CFU / g (L). For the Enterobacteria variable, LRP effect (P <0.0127) was observed, with a minimum time point of 5.01 (R) minutes in the minimum contamination of 0.33 CFU / g (L). For the variable Fungi was found LRP effect (P <0.0071), with a minimum time point of 5.44 (R) minutes in the minimum contamination of 2.38 CFU / g (L). The viral step has not yet been completed. CONCLUSIONS - Data show a reduction in the count of inoculated agents. This reduction occurs at different times according to the agent in question, thus fulfilling the purpose of sanitizing the shaving.
MEMBROS DA BANCA:
Interno - 1760760 - CARLOS EDUARDO NOGUEIRA MARTINS
Interna - 1046884 - ELIZABETH SCHWEGLER
Interna - 2277465 - FABIANA MOREIRA
Interno - 2648941 - IVAN BIANCHI
Presidente - 1085177 - VANESSA PERIPOLLI