Banca de DEFESA: ADRIANA CARLA BALBINOT

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ADRIANA CARLA BALBINOT
DATA : 26/07/2019
HORA: 09:30
LOCAL: Sala 04 do bloco C1 de salas de aula da Medicina Veterinária, do IFC Campus Concórdia
TÍTULO:

Prevalence of Shiga-toxigenic Escherichia coli in pork cuts and offal from slaughtered pigs in the West of Santa Catarina state

PALAVRAS-CHAVES:

Pathogen; stx; swine meat; public health.

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Shiga-toxigenic Escherichia coli (STEC) is recognized as an emerging pathogen group and has become a challenge to public health. STEC strains have virulence factors, such as stx and eae,which characterize them as pathogenic, whereas the determination of their serogroups allows predicting their zoonotic potential. In this sense, the objective of this study was to verify the prevalence of STEC strains in pork cuts and offal, produced in agroindustry in the West of Santa Catarina, detecting the virulence genes and determining the important serogroups for public health. For this, 740 samples of different cuts (steak, shoulder, ham and rib) and offal (heart and liver) were evaluated. Samples were submitted to real-time PCR for the detection of stx1, stx2 and eae genes. Subsequently, samples contaminated with STEC strains were screened by real-time PCR for the following serogroups: O26, O45, O103, O111, O121, O145 and O157: H7. In total, 142 samples (19.14%) were found with virulence factors, being 93 (12.53%) for eae, 20 (2.70%) for stx1/stx2 and 29 (3.91%) for stx1/stx2/eae. Two (0.27%) out of 49 (6.6%) samples, contaminated by STEC strains, were positive for serogroup O121. The low prevalence of potentially zoonotic STEC (0.27%), observed in different swine products, demonstrates that this protein offers a low risk of infection of this pathogen to the last consumer.

 

MEMBROS DA BANCA:
Externa à Instituição - Marisa Macagnan
Presidente - 1756086 - DIOGENES DEZEN
Interna - 2408296 - SORAYA REGINA SACCO
Externo à Instituição - WANDERSON ADRIANO BISCOLA PEREIRA