Banca de DEFESA: TALITA CARINA BOGONI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : TALITA CARINA BOGONI
DATA : 26/07/2019
HORA: 13:30
LOCAL: Sala 04 do bloco C1 de salas de aula da Medicina Veterinária, do IFC Campus Concórdia.
TÍTULO:

Molecular detection of Leptospira interrogans in cattle slaughtered in Concórdia – SC.

PALAVRAS-CHAVES:

Bovine, PCR, Leptospirosis

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Leptospirosis is a important disease of a zoonotic character, caused by bacteria of the genus Leptospira. In cattle, it is between the more frequently reproductive diseases, bring forth economic losses caused by infertility, abortions, weak calves and udder complications. However, the diagnosis of the disease presents some complications, such as: nonspecific clinical signs, fastidious process of isolation/classification of pathogenic strains and need for specialized laboratory for performing the sorological microscopic agglutination test (MAT), the latter being considered de gold standard for the diagnosis of leptospirosis. An alternative is the use of molecular biology techniques, wich have as advantages, the speed, the possibility of detection of this pathogen in diferente stages of the disease and its availability in a large number of laboratories. In this sense, this study aimed to estimate the prevalence of pathogenic leptospira strains in 178 cattle slaughtered in Concordia, SC. For this, at the time of slaughter, biological samples were collected from these animals, they were submitted to a DNA extraction protocol and subsequently, to Polymerase Chain Reaction (PCR). Of the samples that presented amplification, the following techniques were performed: MAT, Warthin-Starry and comparative histopathology. In PCR, seven (3,9%) urine samples showed specific amplification; whereas in the renal tissue no positive samples was obtained. Of the positive animals in PCR, two had anti-Leptospira antibody titers, all of them presented instestitial nephritis and in none was it possible to detect the presence of spirochete through silver staining. These results indicate that the pathogen is circulating in the region and chronic carriers, infected and sorologically negative animals, may be contributing to the transmission of the disease. Therefore, actions aimed at the accurate diagnosis of the agent may contribute to the control and prevention of the disease.

MEMBROS DA BANCA:
Presidente - 1756086 - DIOGENES DEZEN
Externo à Instituição - FELIPE GERALDO PAPPEN
Externa à Instituição - FERNANDA ZORDAN FONTANA
Interna - 2408296 - SORAYA REGINA SACCO