Banca de DEFESA: ELIETE GRIEBELER

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : ELIETE GRIEBELER
DATA : 25/07/2019
HORA: 13:30
LOCAL: Sala 04 do bloco C1 de salas de aula da Medicina Veterinária, do IFC Campus Concórdia
TÍTULO:

Molecular detection of Mollicutes and Mycoplasmaspp. in milk samples, with absence of growth in conventional microbiological isolation and from bovines with mastitis

 

PALAVRAS-CHAVES:

Intramammary infection; Mycoplasmosis; Polymerase Chain Reaction; Dairy Cattle.

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Mastitis is a costly disease, highly prevalent in dairy farms, resulting in high production costs and leading to significant economic losses. The control of mastitis is based on the diagnosis of the etiologic agent. Among the agents responsible for outbreaks of the disease, the genus Mycoplasma stands out, which needs specific diagnostic techniques for its detection. In Santa Catarina, data on the presence of this microorganism in milk are not available. In this regard, the study aiming to detect, through a PCR assay, the presence of Mollicutes and Mycoplasma spp., in milk samples negative to conventional microbiological isolation from cows with mastitis. For this purpose, a total of 187 milk samples of mastitis cases from dairy herds of the State of Santa Catarina were used. The samples were submitted to DNA extraction protocols using the phenol-chloroform method, thereafter the PCR was performed to identify the Mollicutes class. The positive samples were submitted to a new PCR reaction to the genus Mycoplasma. Subsequently, DNA fragments obtained in the genus-specific PCR amplification were cloned, the recombinant clones were sequenced and the DNA sequences obtained were subjected to homology analysis. Of the analyzed samples, 1.6% were positive for Mollicutes and 1.1% presented amplification for the genus-specific PCR, but the sequencing did not verify homology with the genus Mycoplasma. Therefore, the presence of this bacterial class in Santa Catarina state was reported, allowing the inclusion of these microorganisms in the etiological suspicion of mastitis.

MEMBROS DA BANCA:
Externa à Instituição - Franciele Rampazzo Vancin
Presidente - 1756086 - DIOGENES DEZEN
Interna - 2408296 - SORAYA REGINA SACCO
Externo à Instituição - WANDERSON ADRIANO BISCOLA PEREIRA