Banca de DEFESA: HENRIQUE LARSEN BRUNOW VENTURA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : HENRIQUE LARSEN BRUNOW VENTURA
DATA : 25/07/2019
HORA: 14:00
LOCAL: Imunova Análises Biológicas - Sala de Reunião 1
TÍTULO:

Inputs to quick test for bovine mastitis diagnosis

PALAVRAS-CHAVES:

Polyclonal antibodies, dairy cattle, dairy control, sensitivity, specificity.

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Milk and its products are invaluable sources of nutrients, but their production may be affected by mastitis. Therefore, mastistis detection is crucial for resolution of this problem in dairy cattle herd. Definitive diagnosis is reached through milk bacterial isolation. Since diagnostic sensitivity is a critical point for the microbiological method, false negative results interfere with the effectiveness of the diagnosis. Microbiology is qualitative, although considered definitive, may be affected by variables that can make results confusing. Currently used farm tests may also result on false negatives. Therefore, the development of an improved, rapid and low-cost method with better specificity and sensivity, might be important. The goal of the project was to create a kit with polyclonal IgY antibodies obtained from yolks of hen eggs previously immunized against mastitis pathogens. This test will be developed from bacteria derived from milk of mastitis-suspected cows, followed by production of antibodies against these agents and verification of the diagnostics parameters, comparing with reference methods. Milk bacteria were cultivated in vitro. genera of the isolated bacteria were confirmed by sequencing RNA 16R gene . Inactivated agents were inoculated in hens on egg production stage. The hen sera was collected and screened against each bacteria through agglutination test, AGID and tested through Enzyme-linked Immunosorbent Assay (ELISA). When potential applicability was found, the corresponding eggs were used for IgY extraction and competition ELISA. On base 10 dilution ELISA, anti-S. aureus sera had a stronger reaction than the pre-immune sera and than the blank control, which means its specificity.Polyclonal antibodies against S. aureus were extracted from egg yolk and tested by competition ELISA in farm milk samples. After confirmation by linear regression to the standard control of the test, it was verified that anti-S. aureus IgY might be reliable inputs to be used in the development of a prototype kit.

MEMBROS DA BANCA:
Externa à Instituição - Josir Laine Aparecida Veschi
Presidente - 047.057.669-38 - BRENO CASTELLO BRANCO BEIRÃO - UFPR
Interno - 1756086 - DIOGENES DEZEN
Interna - 1046884 - ELIZABETH SCHWEGLER