Banca de DEFESA: ALAN SAVARIZ
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.DISCENTE : ALAN SAVARIZ
DATA : 10/07/2019
HORA: 15:00
LOCAL: Instituto Federal Catarinense Campus de Concórdia
TÍTULO:
Salmonella enterica in fluvial environments: evaluation of the presence and resistance to antibiotics
PALAVRAS-CHAVES:
Aquatic environment; Water pollution; Surface water
PÁGINAS: 53
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:
Agricultural activities and urban effluents are the main sources of surface water contamination by Salmonella enterica. When released into water bodies they can survive for long periods of time and be transported over long distances, transforming aquatic ecosystems into reservoirs. The objective of this study was to evaluate the contamination of Capinzal River by Salmonella enterica and to trace its resistance profiles to antibiotics. The Rio Capinzal is located in the municipality of Capinzal (27 ° 20'37 "S, 51 ° 36'43" W), Santa Catarina, Brazil. Water and sediment samples were collected seasonally in 2016 and 2018 at six points along the river: three points in the rural area and three points in the urban area of the municipality. The points were distributed along the route of the river to cover from the source to near its mouth. The methodology used to analyze the presence of Salmonella spp. is described in ISO 6579: 2002 / Amd.1: 2007 and ISO 6579-1: 2017. To define the antibiotic resistance profiles, the Kirby & Bauer disc diffusion method was used using Muller Hinton Agar. A total of 54 water and 54 sediment samples were collected, totaling 108 samples. Of the 108 samples collected, 37 (34.6%) were positive for Salmonella enterica. In the rural areas of the 54 samples collected, 6 (11.11%) presented S. enterica contamination. The points of the urban area had a greater number of positive samples, of the 54 water and sediment samples collected 31 (54.4%) presented Salmonella enterica. This result indicates that the contamination by Salmonella enterica begins in the rural area and intensifies in the urban area of the municipality. Seven serovars were isolated along the river, the most frequent being S. Infantis (23,53%), S. Orion (23,53%), S. Javiana (23,53%), S. Senfterberg (11,76%), S. Montevideo (5,88%), S. Heidelberg (5,88%), S. enterica subsp. enterica (O: 6,8) (5,88%). With respect to antibiotic of the 37 isolated cultures, one (2.7%) presented resistance to ampicillin, one (2.7%) to tetracycline and three (8.1%) to gentamicin. One (2.7%) presented resistance to meropenem and cefepime. Five (13.51%) were resistant to ampicillin, sulfamethoxazole + trimethoprim, amoxicillin + clavulanate, tetracycline and chloramphenicol. Another two (5.4%) were resistant to all five antibiotics mentioned above and were still resistant to gentamicin and therefore resistant to the six drugs tested. The high frequency of isolates and observed differences in the resistance profiles of Salmonella cultures present in the water body pose potential risks of contamination of the entire trophic chain, river environment and human population.
MEMBROS DA BANCA:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Externa à Instituição - FERNANDA MAURER DAGOSTINI - PUC - RS
Interno - 1248656 - PAULO MAFRA DE ALMEIDA COSTA
Interna - 1081425 - TEANE MILAGRES AUGUSTO GOMES