Evaluation of poultry litter treatments against Clostridium perfringens, enterobacteria, and coccidiosis in dark house and conventional aviaries of broiler chickens
Clostridiosis, Enterobacteriaceae, aviary litter.
Due to the poultry trend to migrate from conventional to dark house system, the aim of this work was to evaluate two treatments of broiler chicken litters against Clostridium perfringens, enterobacteria, and coccicidiosis, and also compare both aviary systems. Forty litter samples were divided in four groups: T1 - dark house with quicklime; T2 - dark house with fermentation and quicklime; T3 - conventional with quicklime; T4 - conventional with fermentation and quicklime. All samples were collected one day before slaughter and five days after treatment, resuspended, dilluted and plated in selective media for enterobacteria and C. perfringens. The bacterial load in pre-treated litter was similar between dark house and conventional systems. Groups treated with quicklime showed a significant reduction (p <0.05) of enterobacteria in both systems. Reduction of C. perfringens was only observed in group T4. However, all groups showed low values of C. perfringens before treatment, as well as reduced percentage of samples with α toxin gene (cpa), identified by PCR. All groups showed very low values of Eimeria spp. in litter before and after treatment, which deprived comparing treatments and aviary systems. In conclusion, quicklime treatment (500g/m2) is suitable against enterobacteria, allowing the reuse of litters with low risk of enteric diseases in broiler chickens.