Banca de QUALIFICAÇÃO: HENRIQUE LARSEN BRUNOW VENTURA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
DISCENTE : HENRIQUE LARSEN BRUNOW VENTURA
DATA : 07/12/2018
HORA: 11:00
LOCAL: Instituto Federal Catarinense Campus Araquari, Prédio D, sala 302b
TÍTULO:

Rapid and portable prototype test for bovine mastitis diagnosis.

PALAVRAS-CHAVES:

Polyclonal antibodies, dairy cattle, dairy control, sensitivity, specificity.

PÁGINAS: 45
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Milk and its products are invaluable sources of nutrients, but their production may be affected by mastitis. Therefore, mastistis detection is crucial for resolution of this problem in dairy cattle herd. Definitive diagnosis is reached through milk bacterial isolation. Since diagnostic sensitivity is a critical point for the microbiological method, false negative results interfere with the effectiveness of the diagnosis. Microbiology is qualitative, although considered definitive, may be affected by variables that can make results confusing. Currently used farm tests may also result on false negatives. Therefore, the development of an improved, rapid and low-cost method with better specificity and sensivity, might be important. The goal of the project was to create a kit with polyclonal IgY antibodies obtained from yolks of hen eggs previously immunized against mastitis pathogens. This test will be developed from bacteria derived from milk of mastitis-suspected cows, followed by production of antibodies against these agents and verification of the diagnostics parameters, comparing with reference methods. Milk bacteria were cultivated in vitro. genera of the isolated bacteria were confirmed by sequencing RNA 16R gene . Inactivated agents were inoculated in hens on egg production stage. Hen sera was collected and tested against each bacteria through agglutination test and Enzyme-linked Immunosorbent Assay (ELISA). Anti Staphylococcus sp. sera did not agglutinate with specificity, anti Streptococcus sp and anti S. uberis sera agglutinated up to the dilution of 1:8. Anti S. aureus sera agglutinated up to 1:2 and, on a base 10 dilution ELISA, had stronger reaction than the pre-immune sera and than the blank control, which indicates high specificity. Thus, polyclonal antibodies against S. aureus might be the main reagents to be used on the prototype kit development. On the next stage, anti S. aureus sera will be tested on farm milk samples in order to confirm its sensivity.

MEMBROS DA BANCA:
Presidente - 047.057.669-38 - BRENO CASTELLO BRANCO BEIRÃO - UFPR
Interno - 1760760 - CARLOS EDUARDO NOGUEIRA MARTINS
Interno - 1046884 - ELIZABETH SCHWEGLER
Interno - 2277465 - FABIANA MOREIRA
Interno - 2648941 - IVAN BIANCHI
Interno - 1430573 - MIGUELANGELO ZIEGLER ARBOITTE
Interno - 1830781 - ADOLFO JATOBA MEDEIROS BEZERRA
Interno - 1085177 - VANESSA PERIPOLLI