Methodology for bioconservation and viability of umbilical cord of swine (sus scrofa domesticus) by microbiological and histological analysis
Membranes; Chlorhexidine; Conservation; Histology.
The objective of the present work was to analyze effective and biocompatible methods of conservation of umbilical cords collected from newborn piglets, by means of the evaluation of the microbiological contamination of the samples and the preservation of the histological characteristics, seeking to compare conservation results with chlorhexidine based solutions with the results of glycerin 98% and hypersaturated salt solutions used for the same purpose. Were collected umbilical cords from neonate piglets and they were stored in one of the solutions (chlorhexidine aqueous solution, chlorhexidine 2%, chlorhexidine 4%, glycerin 98% or hypersaturated salt) for up to 60 days. Fresh umbilical cords (T0), 15 (T15), 30 (T30) and 60 days (T60) were evaluated in the solution to determine degree of contamination and histological characteristics. Microbiological analysis of the fresh umbilical cords (T0) showed high degree contamination of the samples, which, over the course of the treatment time, was reduced, being sterilized after 60 days. On the histological analysis of the fresh umbilical cords (T0) it was possible to identify a stratified squamous epithelium, which did not undergo significant changes during the treatment time. These results demonstrate that the conservation means studied showed similar responses in the conservation of swine umbilical cords, all of which are suitable for this purpose after 60 days of storage.