Banca de DEFESA: CAROLINE REICHEN

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : CAROLINE REICHEN
DATA : 23/07/2018
HORA: 09:00
LOCAL: Embrapa Suínos e Aves
TÍTULO:

Evaluation of the efficiency of vaccination use to reduce the prevalence of Salmonella sp. in pig slaughter

PALAVRAS-CHAVES:

Field trial; subunit vaccine; fattening; seroprevalence, shedders; carriers

PÁGINAS: 57
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

Among the foodborne diseases, Salmonellosis is the most frequent, being controlled in the products of animal origin. Even though quality control programs should be implemented in the slaughter and meat processing, measures driven to the herds could contribute to decrease carcasses contamination. The purpose of this field trial was to evaluate the use of a subunit vaccine, based on secondary antigens, broad-spectrum among Salmonella serovars, in reduction of seroprevalence and prevalence of Salmonella carriers in mesenteric lymph nodes and shedders at slaughter. The experimental unit was a batch of fattening pigs, being chosen randomly 10 finishing farms to place the control group (GC) and 10 for the vaccinated group (GV). Initially, 16 nurseries were chosen to carry out the first vaccination and supply the finishing farms, being 8 for the GC and 8 for the GV. The vaccine was orally supplied, 02 mL by animal at four different ages. The first dose was on the second day of nursery, the second dose 14 days after the first, the third dose after 30 days of fattening and the fourth dose 21 days prior slaughter. The animals remained on the fattening for 110 days on average and slaughtered at 175 days old. Blood samples were collected in the first week of fattening and at slaughter (n=30/batches). Mesenteric lymph nodes (n=30/batches) and feces (n=20/batches) were collected at slaughter. The serum was submitted to ELISA test and lymph node and feces samples submitted to the isolation of Salmonella (ISO 6579). In addition, the quantification of Salmonella in feces was performed by the most probable number technique. There was no statistical difference between the groups (p> 0.05), both for seroprevalence (% of positive pigs) and for the intensity of the serological reaction measured by the variability in the value of optical density. Also, the vaccination did not reduce the prevalence of carriers in mesenteric lymph nodes and Salmonella shedders in feces, as well as their quantity shedding.

MEMBROS DA BANCA:
Interno - 1756086 - DIOGENES DEZEN
Presidente - 564.081.370-91 - JALUSA DEON KICH - EMBRAPA
Externo à Instituição - MARISA RIBEIRO DE ITAPEMA CARDOSO - UFRGS