Banca de DEFESA: JULIANA GRANDI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
DISCENTE : JULIANA GRANDI
DATA : 12/07/2018
HORA: 14:00
LOCAL: Sala 5 do bloco de salas de aula do curso de Medicina Veterinária do Instituto Federal Catarinense
TÍTULO:

In vitro antimicrobial activity of garlic (Allium sativum L.) aqueous extract against microorganisms isolated from external otitis in dogs

PALAVRAS-CHAVES:

otopathy; plant; microbial resistance; coagulase positive Staphylococcus; Malassezia pachydermatis.

PÁGINAS: 60
GRANDE ÁREA: Ciências Agrárias
ÁREA: Medicina Veterinária
RESUMO:

External otitis is an inflammatory process that involves the external auditory canal, with high prevalence in small animals’ veterinary medicine.  Treatment has been difficult, due its multifactorial etiology represented by primary, predisposing and perpetuating factors. Infectious agents as coagulase positive Staphylococcus (CPS) and Malassezia pachydermatis (M. pachydermatis) are examples of perpetuating factors. Actually, the microbial resistance to conventional drugs represents an aggravating process on this disease. In this case, the use of plants with medicinal properties, as garlic (Allium sativum L.) may be considered as part of therapeutically measures. Thus, the objective of the present study was to evaluate the in vitro antimicrobial potential of garlic aqueous extract against microorganisms isolated from external otitis in dogs. For this purpose, it were used 10 CPS and 12 M. pachydermatis strains, obtained from Laboratory of Veterinary Microbiology of Federal Institute (Santa Catarina State), Concórdia, Brazil. These strains were isolated from canine external otitis and belonged to the lab’s bacteriotheca. For aqueous extract production, the garlic samples were obtained from a commercial distributor. The microdilution technique was used for Minimum Inhibitory Concentration (MIC) evaluations, for both bacterial and yeast. The Minimum Fungicide Concentration (MFC) was evaluated for M. pachydermatis. The garlic aqueous extract inhibited the growth of all isolates. The MIC values against bacterial and fungi isolates ranged from 93.75 to 375mg/mL, and from 5.86 to 46.87mg/mL, respectively. The MFC ranged from 5.86 to 375mg/mL. It was possible to conclude that the garlic aqueous extract presents in vitro antimicrobial activity against the main microorganisms isolated from external otitis in dogs. 

MEMBROS DA BANCA:
Interno - 1988158 - EDUARDO NEGRI MUELLER
Externo à Instituição - Márcia de Oliveira Nobre - UFPel
Interno - 2408296 - SORAYA REGINA SACCO