Evaluation of the antimicrobial activity of garlic (Allium sativum) on microorganisms isolated from otitis externa cases in dogs
Otopathy, Plant, Microbial resistance
Otopatias are frequent in companion animals and often difficult to treat, since there is a great variety of perpetuating agents. Many of these microorganisms are resistant to the drugs available in the market. Considering the high casuistry of otitis externa in dogs, the resistance of microorganisms to allopathic drugs and the increase of relapses, it is necessary to find alternative treatments for this disease. Therefore, the objective was to evaluate the in vitro antimicrobial potential of garlic against microorganisms isolated from cases of external otitis in dogs. The aqueous extract of garlic was obtained using the methodology of Venâncio (2010) with modifications. Serial dilutions of 75 to 0.15 g/mL were performed for bacterial MIC and 37.5 to 0.07 g/mL for fungal MIC. Ten samples of Staphylococcus coagulase positive and twelve of Malassezia pachydermatis obtained from clinical isolates of canine otitis externa were processed in the diagnostic routine of the Laboratory of Veterinary Microbiology of the Instituto Federal Catarinense - Campus Concórdia. To obtain the minimum inhibitory concentration (MIC), the sensitivity test was used by dilution in broth, according to Sarker et al. (2007). Regarding yeasts, for the determination of MIC and minimum fungicidal concentration (CFM), the test was adapted according to Santin et al. (2014). All isolated were inhibited. Inhibition was observed from the concentration 9.37 g / mL for bacteria and 0.59 g / mL for yeasts. The partially obtained results confirm the antibacterial and antifungal effect against the studied microorganisms. It can be concluded that the aqueous extract of garlic presents antimicrobial potential against S. coagulase positive and M. pachydermatis isolated from cases of canine otitis externa.