Infection Dynamics of Swine Influenza Virus in Different Herd Formations.
Infection; diagnosis; subtyping; control.
The influenza virus (IAV) is endemic in Brazilian commercial pig farms and causes acute respiratory disease in pigs, which is difficult to treat and control. The objective of this study is to determine the viral subtypes found in herds with different formations. To achieve this, three sets of herds with the same origin from the Swine Breeding Unit (UPD) and three herds from three different origins will be evaluated. Nasal swabs and oral fluid samples were collected for agent detection using the RT-PCR (Real-Time Polymerase Chain Reaction) kit from Integrated DNA Technologies. Samples with CT < 25 will be subjected to viral subtyping according to the laboratory's internal methodology. Sample collection is performed during the weaning week, 10 days after placement in the nursery, and one week before the animals are moved to the finishing stage, where the collected samples are being stored in an Ultrafreezer at -70°C for subsequent RT-PCR analysis.