Banca de QUALIFICAÇÃO: JEAN VITOR BONDAVALLI

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : JEAN VITOR BONDAVALLI
DATE: 01/12/2023
TIME: 10:00
LOCAL: Web Conferência - Google Meet: meet.google.com/cym-bqcs-vot.
TITLE:

IMMUNOCONTRACEPTIVE EFFICACY OF RECOMBINANT VACCINE IN PIGS.

KEY WORDS:

Key words: Escherichia coli; GnRH; Immunocastration; Libido; Imune system; Testosterone.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Gonadotropin-releasing hormone (GnRH) associated with an immune system-stimulating molecule is widely used in contraceptive vaccines. The objective of this study was to evaluate the immunocontraceptive efficacy of a recombinant vaccine consisting of GnRH fused to Escherichia coli LTB toxin in pigs. 32 male pigs aged 60 ± 3.6 days were used, randomly distributed into four groups: Group 1 - animals surgically castrated at three days of age; Group 2 - commercial immunocontraceptive vaccine; Group 3 - purified GnRH vaccine; Group 4 - unpurified GnRH vaccine (bacterin). Animals in groups 2, 3 and 4 received two doses of 2 ml of the immunogen, intramuscularly, on days 41 and 75 of the experiment. The animals were weighed at four times to analyze weight gain (WG) and average daily weight gain (ADG). At 90 days of life, weekly assessments of sexual behavior and libido began. Andrological examinations were performed to evaluate testicular characteristics. To evaluate serum testosterone levels, seroconversion of anti-GnRH antibodies and metabolic markers (thyrotoxin and IGF-1), the animals underwent five blood collections during the period, in addition to being challenged with the administration of GnRH 20 days after the second dose of the immunocontraceptive vaccine. At 105 days of experiment, the animals were slaughtered in a slaughterhouse with a Federal Inspection Service. The data obtained will be analyzed using the Statistical Analysis System software (SAS Inst. Inc., Cary, NC) version 9.3. So far, from the tabulated data, it has been possible to observe by comparing the means that pigs castrated with non-purified GnRH vaccine have higher WG and ADG (119.4 kg and 1137.4 g, respectively). The weekly assessment of sexual behavior and libido showed a greater number of observations of continuous mounting and sexually active animals in the group of males vaccinated with purified GnRH. Likewise, this group showed greater testicular volume in andrological examinations, after immunological castration. With statistical analyses, it is expected to prove the effectiveness of the LTB/GnRH vaccine in the immunocastration of pigs.

COMMITTEE MEMBERS:
Interno - 2613308 - DELANO DIAS SCHLEDER
Interna - 1046884 - ELIZABETH SCHWEGLER
Presidente - 2277465 - FABIANA MOREIRA
Interno - 1810731 - JULIANO SANTOS GUERETZ
Interno - 1755182 - MARIO LETTIERI TEIXEIRA
Interna - 2408296 - SORAYA REGINA SACCO