Banca de DEFESA: JEAN CARLO VOLPATO FACCIN

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : JEAN CARLO VOLPATO FACCIN
DATE: 02/10/2023
TIME: 13:30
LOCAL: Web Conferência - Google Meet: https://meet.google.com/mpa-cdse-jft.
TITLE:

Effect of bacterial resistance on contamination and quality of refrigerated swine semen.

KEY WORDS:

Antimicrobials, resistant microorganisms, artificial insemination, pig farming.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Most swine ejaculates after collection show bacterial contamination and most agents are resistant to frequently used antimicrobials. This work aims to evaluate whether bacteria resistant to antimicrobials used in swine semen extenders are correlated with the decrease in the quality of the inseminating dose during storage. For this, samples of ejaculates approved by the quality system of 5 centers were collected, 6 males per center, in 5 visits with intervals of one month. Of the evaluated water samples, 44% showed bacterial growth with an average of 0.86 ± 0.02 log CFU/mL, with G- Rods being isolated in 66.67%, G- Cocobacilli and Staphylococcus spp in 16.67% of the colonies . After the addition of the semen extender, 19.04% showed growth, with an average of 0.03 ± 0.01 log CFU/mL and only G- Rods and Staphylococcus spp were isolated. (50%). Bacterial growth was identified in 88.66% of pure semen samples; 21.3% of freshly diluted semen samples, 10.66% of samples after 24 hours and 20% of samples after 120 hours. Means of log CFU/ml for the points evaluated above were: 2.07 ± 0.10; 0.16 ± 0.05; 0.09 ± 0.02 and 0.26 ± 0.07, respectively, with the average for pure semen being significantly higher (p < 0.05). Only these results already demonstrate partial antimicrobial capacity of the extender, since the bacteria returned to grow over time in detectable concentrations by in vitro cultivation. Of the tested isolates, 22.45% of the samples were positive for resistance. Thus, it can be concluded that a wide variety of isolates can be found both in ejaculate and in already diluted and bottled semen, emphasizing the need for hygiene during semen collection and processing.

COMMITTEE MEMBERS:
Externa à Instituição - ANA PAULA GONÇALVES MELLAGI - UFRGS
Externo à Instituição - CARLOS CABRAL - PUCMinas
Presidente - ***.965.078-** - MARIANA GROKE MARQUES - EMBRAPA