Epidemiological study of the Gumboro disease virus in vaccinated broilers in the state of Paraná and its relationship with lesions in organs of the immune system.
Bursa of Fabricius; RFLP; Genogroup 4; G15
Gumboro disease virus belongs to the Birnaviridae family and to the Avibirnavirus genus, and this agent continually represents a challenge for world poultry production. In South America, Genogroup 4 (dIBDV) were found in commercial broiler flocks. The present study aimed to carry out an epidemiological study of the Gumboro virus in commercial broiler flocks in the state of Paraná (main chicken producing state in Brazil), genotyping of Gumboro virus strains and to correlate it with lesions found in organs of the immune system and zootechnical results. Were evaluated 30 broiler flocks with an average of 24,949 birds per flock, aged between 18 and 23 days. Five samples of Bursa of Fabricius were collected per flock for virus detection and genotyping. Positive samples for Gumboro virus with RFLP distinct from the vaccine strains were subjected to the sequencing. Five samples of Bursa of Fabricius, thymus, spleen, cecal tonsils and bone marrow were also collected per flock for histopathological analysis. Of the 30 flocks evaluated, in 10 (33%) strains of Gumboro Genogroup 4 (dIBDV) were detected. Lesion at Bursa of Fabricius varied according to the viral strains detected, and it was mild when no virus was detected in the Bursa (negative). No significant alterations were detected in the other evaluated lymphoid organs. Zootechnical data (feed conversion, daily weight gain, production efficiency index and mortality) were worst when field strains from Genogroup 4 was present in the Bursa.