Banca de DEFESA: TAISA VANESSA OLDONI

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : TAISA VANESSA OLDONI
DATE: 08/07/2023
TIME: 08:30
LOCAL: Banca Fechada.
TITLE:

3D Prototyping for Prostheses Incorporating Rationally Synthesized Compounds with Antimicrobial Activity in Application in Veterinary Medicine.

KEY WORDS:

denture-related infections; animal models; 3D printing; bioprinting; Candida spp infections.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

In the veterinary field, 3D printing is being used more frequently in veterinary hospitals, both in the planning of surgeries and in the manufacture of prostheses. With the manufacture of prostheses, through 3D printing, their cost is considerably reduced, being manufactured in a few hours and can be molded according to the needs of the animal. Fungi can exhibit pathogenicity for humans and animals and, depending on the tissue or organ affected, can lead to death. Candida spp. are yeast-like organisms, among the species of the genus, Candida albicans is the species with the highest incidence isolated in dogs and cats. The uncontrolled increase in the use of different broad-spectrum chemotherapeutic agents to treat the most diverse infections is increasingly evident, without identifying the microorganism and its susceptibility. Thus, there is a need to prove sensitivity and safety in the incorporation of rationally synthesized compounds in the manufacture of prostheses. The objectives of this dissertation were: To develop prototypes of biofunctionalized prostheses for use in the area of veterinary medicine containing formulations of rationally synthesized compounds (CRS) known to have antimicrobial activity for efficacy and toxicological tests in ex vivo and in vivo models. Three experiments were evaluated to prove the efficacy and safety of incorporating 8-HQ in the manufacture of prostheses, including the permeation test and histopathological evaluation, which confirmed that it had no topical toxicity. The antifungal activity experiment in loco through the disk diffusion test proved the sensitivity of 8-HQ against strains of Candida spp. in a halo superior to the reference medicine. In the toxicity assessment using the HET-CAM methodology, it showed an irritation index of 4.03, classified as non-irritant. The present study demonstrated the safety of incorporating 8-hydroxyquinolone in the manufacture of gray resin prostheses using 3D technology, with no irritation proven by permeation tests and HET-CAM. In addition, the sensitivity against the strains of Candida ssp tested has been proven.

COMMITTEE MEMBERS:
Externa ao Programa - 2101600 - ANA CAROLINA GONCALVES DOS REIS - nullPresidente - 1755182 - MARIO LETTIERI TEIXEIRA
Externa à Instituição - RUBIA MORES - UNC