Banca de DEFESA: SABINE RIPPEL STAHLHOFER

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : SABINE RIPPEL STAHLHOFER
DATE: 05/12/2022
TIME: 08:00
LOCAL: Web Conferência - Google Meet: https://meet.google.com/onh-mnbm-ujw
TITLE:

Genotypic, phenotypic and pathogenicity evaluation of Salmonella Thompson isolates in the poultry industry.

KEY WORDS:

broiler; molecular characterization; control; epidemiology; antimicrobial resistance

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Salmonella Thompson has been identified in several countries, being one of the main serovars involved in foodborne infections in humans in the European Union. The objective of the study was to evaluate the phenotypic, genotypic and pathogenicity profiles of S. Thompson isolates from the poultry chain of a Brazilian company, aiming at a better understanding of this pathogen. To carry out the experiments, 44 strains from different sectors were selected. The strains were submitted to a susceptibility test against 12 antimicrobial agents. Sulfonamide resistance was 15.91%, and tetracycline resistance was 2.22%. In the genotypic evaluation of the isolates through RT-PCR in real time, genes related to resistance and virulence factors were investigated. ThetA resistance genes sul1 and sul2 were present in most samples (97.73%, 79.55% and 86.36%, respectively). Of the total, 26 (59.09%) had one or more virulence genes. The identification of carbapenemase, colistin and beta-lactamase resistance genes was carried out by the microarray technique, with one strain showing resistance to carbapenamase and the other to ESBL. In addition, the molecular typing of the isolates was performed by the PFGE technique, which showed little genetic variability, forming 5 non-clonal PFGE patterns. One isolate from each cluster was selected for pathogenicity assessment by challenge in commercial birds. The strain of group 3, proved to be the most invasive when compared to the other strains. The information generated in this study helps and improves Salmonella control strategies, showing the need to use molecular analyzes as auxiliary tools in the control strategies of this pathogen in the poultry chain.

COMMITTEE MEMBERS:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Interna - 1081425 - TEANE MILAGRES AUGUSTO GOMES
Externa à Instituição - MARITA VEDOVELLI CARDOZO - UFMG