Banca de DEFESA: MARIANA SANTIAGO GOSLAR

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIANA SANTIAGO GOSLAR
DATE: 24/12/2022
TIME: 13:00
LOCAL: Web Conferência - Google Meet: meet.google.com/mcv-dexd-qmi.
TITLE:

Characterization of Influenza A subtypes in growing and finishing pigs in integration in the South and Midwest region of Brazil.

KEY WORDS:

viruses; RT qPCR; subtyping; diagnosis; respiratory diseases.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The Influenza A virus (IAV) is an RNA virus that causes respiratory diseases and has zoonotic character, and is considered endemic in commercial swine herds in Brazil. Facing several antigenic variations and genetically distinct lineages (subtypes H1N1, H1N2, H3N2) in the country, fast and accurate diagnostic tools are necessary to know the subtypes present in each farm, in order to monitor and control the disease more assertively. This study aims to use RT- qPCR for detection of Influenza A virus and subtyping by identifying mutations in the genes of surface glycoproteins Hemagglutinin and Neuraminidase. A total of 2,052 samples from pigs in various productive phases, collected in six different integration units of an Agroindustry, were analyzed in the Animal Health laboratory, in the nasal swabs and oral fluid, as herd monitoring and lungs from clinical cases, between April and December 2021. The presence of IAV was detected in 17.1% of the samples. The sample type with the highest positivity was oral fluid, where the presence of the virus was detected in all cases. The Carambeí (PR) unit was the most positive for IAV (66.66%). Multipliers showed positivity in half of the samples. Subtypes were identified in 70 of the positive samples, the H3hu subtype was the most found (35.71%) followed by N1pan (31.43%). It is necessary to take into account the low sampling in the units of Dourados (MS) and Carambeí (PR), in addition to the production phase, because sending samples to the laboratory is related to the presence of clinical signs in the farm. However, this study was able to identify the presence of IAV using qPCR RT and subtyping 19.94% of positive samples. It is necessary to conduct further study, through genome sequencing, to evaluate the phylogeny of the most genetically distinct strains and those that it was not possible to identify subtypes.

COMMITTEE MEMBERS:
Externa à Instituição - CARINE KUNZLER SOUZA
Externa à Instituição - LAURA LOPES DE ALMEIDA
Presidente - ***.812.306-** - JANICE REIS CIACCI ZANELLA - EMBRAPA