Banca de QUALIFICAÇÃO: BÁRBARA PRISCILA PEREIRA DA SILVA

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : BÁRBARA PRISCILA PEREIRA DA SILVA
DATE: 02/12/2022
TIME: 09:00
LOCAL: Web Conferência - Google Meet: meet.google.com/hep-fyaf-hah.
TITLE:

Silver nanoparticles in the fight against bovine mastitis caused by Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli.

KEY WORDS:

Nanometals; mammary gland; infection; bacteria; Ruminants.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Bovine mastitis is the most frequent disease in the dairy herd. Among the main microorganisms causing mastitis Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli stand out. The widespread use of broad-spectrum antibiotics has resulted in the development of resistance by numerous bacterial pathogens. Thus, the objective of this work is to test the efficacy of silver nanoparticle in vitro against the bacterial agents mentioned above. The tests being performed in the study are: zone of inhibition, quantification of viable cells in biofilm and plankton cells, toxicity test in bovine eyeball. All stages are in triplicates with 3 repetitions. For the zone of inhibition in Mueller Hinton Agar 7 concentrations are being tested: 62.5mg/ml, 125mg/ml, 250mg/ml, 500mg/ml, 1,000mg/ml, 2,000mg/ml, 4,000mg/ml. The positive control will be with penicillin disc G 10U (Laborclin®) and the negative control with sterilized distilled water. The plates are incubated at 37ºC for 24 hours. There was zone of inhibition greater than 10mm at concentrations of 1,000mg/ml, 2,000mg/ml and 4,000mg/ml, with a minimum injunction concentration of 1,000mg/ml of silver nanoparticle. For the quantification of viable cells in biofilm and plankton cells 3 concentrations from the minimum inhibition concentration are being tested. A repetition of the quantification of viable cells in biofilm and the quantification of plankton cells were performed in the bacteria Escherichia coli and Staphylococcus aureus. Results obtained so far are being promising in the bacterial inhibition of the evaluated agents.

COMMITTEE MEMBERS:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Interno - 2613308 - DELANO DIAS SCHLEDER
Interna - 1046884 - ELIZABETH SCHWEGLER
Interno - 1810731 - JULIANO SANTOS GUERETZ
Interno - 2017813 - RICARDO EVANDRO MENDES
Interna - 2408296 - SORAYA REGINA SACCO