Banca de QUALIFICAÇÃO: ALISSON LEANDRO ANSOLIN

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : ALISSON LEANDRO ANSOLIN
DATE: 02/12/2022
TIME: 15:00
LOCAL: Web Conferência - Google Meet: meet.google.com/hep-fyaf-hah.
TITLE:

Impact of microbiota, age and housing system of the boar pig on bacterial contamination and productivity of semen doses.

KEY WORDS:

semen bacteriology, swine semen, preputial fluid, preputial washing.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

A considerable number of studies suggest the negative impact of bacterial contamination on swine semen doses. The process in semen production centers is focused on producing doses free of any bacteria or mitigating their appearance, using as alternatives the increased use of antibiotics or constant changes of diluents, without understanding the bacterial load or the types of bacteria that are part of the local microbiota. The knowledge of the impact of the microbiota of the boar in the contamination of the ejaculates can bring alternatives for the control of the contamination, thus improving the fertility of the dose and minimizing the use of antimicrobials in the inseminating doses, and can have an impact on the reduction of antimicrobial resistance genes in the swine industry. Thus, this study has the objective of evaluating the effect of the microbiota of the boar (intestinal and preputial) and factors that can modulate it, such as place of accommodation, age of the boar, use of antimicrobials, preputial washing in the bacterial contamination of ejaculates and the consequence of this contamination on the reproductive potential (in vitro) of doses stored for 5 days under refrigeration. In experiment 1, 24 boars of the same genetic line were used, divided into two groups according to birth origin (origin 1, n=12 and origin 2, n=12). The boars were kept for 30 days in a quarantine and then transferred to two semen collection barns in the same semen production unit. Preputial swab, prepucial fluid, and pure ejaculate collections were collected, with 2 collections in the quarantine period and another 5 collections in the main barns. Seminal analysis, quantification, and bacterial isolation of the collected samples were performed. In experiment 2, 72 boars of the same genetic line and similar age were used and divided into two groups, without preputial washing (group 1, n=36) and with preputial washing (group 2, n=36). The boars were kept in the same barn and were collected for 6 weeks. The preputial washing process in group 1 was made with 0.9% sodium chloride saline solution with infusion into the diverticulum and preputial canal. For both treatments, preputial swab samples, pre-spermatic portion, pure ejaculate, and diluted doses were collected from all the boars. It is expected that there will be a difference in the bacterial contamination of the ejaculates as well as in the productivity of the doses produced according to the origin and age of the boars, as well as it is expected that the effect of the preputial washing with saline solution will be beneficial in relation to the reduction of the contamination level of the ejaculate.

COMMITTEE MEMBERS:
Interno - 2613308 - DELANO DIAS SCHLEDER
Interna - 1046884 - ELIZABETH SCHWEGLER
Presidente - 2648941 - IVAN BIANCHI
Interno - 1810731 - JULIANO SANTOS GUERETZ
Interno - 2017813 - RICARDO EVANDRO MENDES
Interna - 2408296 - SORAYA REGINA SACCO