Effect of bacterial resistance on contamination and quality of cooled swine semen.
Antimicrobials, resistant microorganisms, insemination, swine culture.
The majority of swine ejaculates after collection have bacterial contamination in addition to the majority of agents having resistance to frequently used antimicrobials. This work aims to evaluate whether bacteria resistant to antimicrobials used in swine semen extenders have a correlation with the decrease in the quality of the insemination dose during storage. There will be 5 visits to 5 swine semen collection centers to collect material, including pure semen, ultrapure water used in the preparation of extenders, semen extenders and samples of the insemination dose. The insemination dose samples will be kept in storage between 15 and 18ºC, during storage the dose will be aliquoted and this portion will be sent to bacterial culture on days 1 and 5 after processing at the central and at the same time will be evaluated for semen quality and fertility. The samples collected will be sent for cultivation and identification of bacterial species and later will be tested for their susceptibility to various antimicrobials. Crops that show resistance will undergo a PCR test to identify the gene responsible for resistance. Data will be submitted to the Shapiro-wilk normality test, those with normal distribution will be submitted to Pearson's correlation test, or data with abnormal distribution will then be applied to the Spearman's correlation test.