Banca de DEFESA: MAURÍCIO EDUARDO MEZAROBA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MAURÍCIO EDUARDO MEZAROBA
DATE: 24/08/2022
TIME: 14:00
LOCAL: IFC - Campus Concórdia e https://meet.google.com/osu-cmvq-ewf.
TITLE:

USE OF THE VORTEX AS DISSOCIATOR OF PLATELET AGGREGATES IN PSEUDOTHROMBOCYTOPENIA IN SMALL ANIMALS.

KEY WORDS:

Clinical pathology, hematology, thrombocytopenia, canines, felines.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Pseudothrombocytopenia is a false decrease in platelet count, which occurs due to interference in blood collection and inadequate action of the anticoagulant, having as main cause the formation of platelet aggregates. This represents an important problem in the canine and feline clinic, as it can result in misdiagnosis, unnecessary request for laboratory tests and totally inadequate therapeutic conduct. The aim of this study was to evaluate the use of a vortex shaker as an alternative for laboratory resolution of pseudothrombocytopenia in these species. The project was carried out at the Amigovida Laboratory – Veterinary Analysis (Videira, Santa Catarina) with samples collected by the partner veterinary clinics. Samples of tubes containing anticoagulante ethylenenitrilotetraacetate potassium (EDTA) were selected (canines n=12 and felines n=16), which showed a decrease in platelet count, performed in a cell conter apparatus, by bioimpedance, in addition to the presence of platelet aggregates , evaluated during observation of stained blood smears. Platelets were quantitatively evaluated pre and post treatment with the shaker by automated counting by bioimpedance in a veterinary hematology analyzer SDH-3-VET (Labtest®) and by mean estimation of platelet count per immersion field (1000X magnification). The treatment was carried out by vortexing (Na3600, FORTECIENTÍFICA®) at 3600 revolutions per minute (rpm) for three minutes, followed by homogenization of the sample to make a smear and recount by electrical bioimpedance. The data obtained underwent descriptive analysis and normality test (Shapiro Wilk) using the Minitab® program and for parametric variables, Student's t test was used and for non-parametric variables, the Mann-Whitney test. The analysis resulted in a significant difference between the values of platelets by field and by bioimpedance pre-post treatment (p<0.05), obtaining higher values in all patients evaluated after treatment with agitation, with mean platelets within the values of reference for the species evaluated. It is concluded that the use of a vortex mixer in the proposed protocol associated with the evaluation of platelets by bioelectrical impedance or platelet estimation per field is able to reduce the incidence of pseudothrombocytopenia in felines in approximately 62.5% of felines and in 75% of canines and decrease the underestimated count range due to the presence of platelet aggregates in 100% of the animals. However, the use of the stirrer in this rotation and time should not be used as the only evaluation parameter for pseudothrombocytopenia in domestic animals, considering that even after using the technique there was the presence of platelet aggregates in blood smears, showing that the dissociation is not total, although it minimizes false diagnoses and helps the clinician in a more appropriate therapeutic approach.

BANKING MEMBERS:
Externa à Instituição - ANGELA PATRICIA MEDEIROS VEIGA - UFSC
Externa à Instituição - BIANCA PAOLA SANTAROSA - UFVJM
Presidente - 2408296 - SORAYA REGINA SACCO