Banca de QUALIFICAÇÃO: MARIANA SANTIAGO GOSLAR

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : MARIANA SANTIAGO GOSLAR
DATE: 03/12/2021
TIME: 15:30
LOCAL: Web Conferência - Google Meet - meet.google.com/xzj-fecs-daj.
TITLE:

Characterization of Influenza A subtypes in growing and finishing pig in integration of the south region of Brazil.

KEY WORDS:

Virus, RT-qPCR, Subtyping, Diagnosis, Respiratory diseases.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The Influenza A virus (IAV), although considered endemic in commercial swine herds, still has little active surveillance of the infection in Brazil. Given this gap, and the various antigenic variations and genetically distinct lineages (subtypes H1N1, H1N2, H3N2) of the virus circulating in the country, fast and accurate diagnostic tools are needed to know the subtypes present in each flock, in order to monitor and control the disease more assertively. A total of 2.302 nasal swab samples from nursery and finishing pigs will be analyzed, where it is expected that the RT-qPCR will be able to identify and subtype a large part of the samples and identify a wide variety of recombinant Influenza A viruses in the pig herds in different regions of the country, within the integration of Seara Alimentos. Initially, RT-qPCR analysis was performed for detection and subtyping of Influenza A virus in swine. Subsequently, it will be associated with the genomic sequencing of the most genetically distinct lineages or not identified by RT-qPCR. The partial results of the 1035 samples analyzed through the screening RT-qPCR show that there are 159 active cases until the month of October, where 96 samples are considered positive and eligible for the subtyping RT-qPCR step. IAV infection rates have so far been similar between the coldest and warmest months, not showing seasonality.

BANKING MEMBERS:
Interno - 2613308 - DELANO DIAS SCHLEDER
Interna - 1046884 - ELIZABETH SCHWEGLER
Presidente - 586.812.306-97 - JANICE REIS CIACCI ZANELLA - EMBRAPA
Interno - 2017813 - RICARDO EVANDRO MENDES
Interna - 2408296 - SORAYA REGINA SACCO