Banca de DEFESA: EDUARDO DA SILVA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : EDUARDO DA SILVA
DATE: 03/12/2021
TIME: 09:00
LOCAL: Web Conferência - Google Meet -meet.google.com/azo-fetk-ndj.
TITLE:

Evaluation of the ideal concentration of 2-Phenoxyethanol and essential oil of Eucalyptus globulus as an anesthetic agent and its effects on stress parameters in Rhamdia quelen.

KEY WORDS:

1,8 cineol; 2-Phe; Anesthesia; Cortisol.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

In aquaculture, fish are routinely manipulated, causing physiological stress responses that can cause immunosuppression, growth retardation, pain and even death in the animals. With the need to reduce these adversities in management, anesthetics are used. The objective of this work was to evaluate the ideal concentration (CI) and its effects on the stress responses of Rhamdia quelen submitted to two anesthetics: 2-phenoxyethanol (2-Phe) and essential oil of Eucalyptus globulus (EEO) in different articles. Article 1: Chemical profile analysis of EEO showed that 1,8-cineole is the main component. Then, two experiments were carried out. I) IC Assessment: Four concentrations (600, 650, 700 and 750 μL L-1) were tested. As OEE concentrations increased, induction times reduced, however, it did not influence recovery times. EEO was considered an effective anesthetic in R. quelen (128.65 ± 40.42 g) with a CI of 700 μL L-1 (induction time 2.74 ± 0.67 min and recovery 2.26 ± 1.09 min). During this experiment, there was no mortality. II) Stress assessment: Fish were divided into three groups: control (baseline values, no anesthetic); eugenol (50 mg L-1) and EEO (700 μL L-1). In this assay, EEO inhibited the primary stress response (cortisol did not increase). However, plasma glucose and lactate levels increased. Meanwhile, eugenol kept all stress indicators the same as the control group. Therefore, although EEO has adequate anesthetic characteristics, it should be used with caution, as it did not prevent changes in plasma glucose and lactate levels in R. quelen. Article 2: Two experiments were carried out. I) IC Assessment: Seven concentrations of 2-Phe were tested: 300, 400, 500, 550, 600, 650 and 700 μL L-1. Increasing 2-Phe concentrations, induction times decreased, while recovery times increased. The 2-Phe IC for anesthetizing R. quelen (126.47 ± 41.14 g) was 700 μL L-1 (induction 2.98 ± 0.34 min and recovery time 2.75 ± 0.35 min). II) Stress assessment: Fish were divided into three groups: Control (baseline values, no anesthetic); 2-Phe (700 μL L-1); and Eugenol (50 mg L-1). Plasma cortisol, glucose and lactate levels were measured in fish as stress indicators at two different times: time zero (T0 = anesthetized) and time one (T1 = one hour after anesthesia). The animals in the 2-Phe and Eugenol groups showed a significant increase in glucose values when compared to the control group. Plasma lactate at T0 in the Eugenol and 2-Phe groups did not increase when compared to the control group; however, at T1, a significant increase was observed for both anesthetics. No significant differences were found in plasma cortisol levels between treatments and times. Therefore, 700 μL L-1 of 2-Phe can be considered a suitable anesthetic for R. quelen.

BANKING MEMBERS:
Externo à Instituição - BERNARDO BALDISSEROTTO - UFSM
Interno - 2613308 - DELANO DIAS SCHLEDER
Presidente - 2356802 - ROBILSON ANTONIO WEBER