Banca de QUALIFICAÇÃO: SABINE RIPPEL STAHLHOFER

Uma banca de QUALIFICAÇÃO de MESTRADO foi cadastrada pelo programa.
STUDENT : SABINE RIPPEL STAHLHOFER
DATE: 03/12/2021
TIME: 08:30
LOCAL: Web Conferência - Google Meet - meet.google.com/xzj-fecs-daj
TITLE:

Genotypic, phenotypic and pathogenicity evaluation of Salmonella Thompson isolates in the poultry industry.

KEY WORDS:

Broilers; molecular characterization; control; epidemiology; antimicrobial resistance.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Salmonella enterica subspecie enterica serovar Thompson has been identified in several countries, being one of the 20 main serovars involved in foodborne infections in humans in the European Union. In addition, this serovar has been showing resistance to antimicrobials of interest in human and animal health, thus confirming the importance of investigating factors that contribute to this. It is known that biosecurity measures are important for the control of the agent, however, having understanding about the virulence genes, antimicrobial resistance, genetic profile and its pathogenicity can contribute to its prevention and control. The aim of the study was to evaluate the genotypic, phenotypic and pathogenicity profile of several Salmonella Thompson isolates from the poultry chain of a Brazilian company, thus seeking a better understanding of this pathogen. To carry out the experiments, 44 strains from different agricultural sectors were selected. The strains were subjected to susceptibility testing against 12 antimicrobial agents. The number of sensitive isolates (84.09%) was higher than that of resistant isolates (15.91%). Resistance to sulfonamide was 13.63%, and tetracycline was 2.22%. In the genotypic evaluation of the isolates, the genes related to resistance to tetracyclines tetA, tetB, tetC and the sulfonamides sul1, sul2 and sul3 and the virulence factors iron, sipA, lpfC, sopE and spvB were investigated through real-time RT-PCR . In addition, the molecular typing of the isolates was performed using the PFGE technique, which showed little genetic variability among the 44 isolates, forming 5 non-clonal PFGE patterns. Of the 5 patterns, clusters C, D and E have only 1 isolated. Cluster A has 11 clonal isolates and cluster B has 30 clonal isolates. After genetic and phenotypic analysis, 4 isolates were selected for pathogenicity evaluation through the inoculation of the agent in birds.

BANKING MEMBERS:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Interno - 2613308 - DELANO DIAS SCHLEDER
Interna - 1046884 - ELIZABETH SCHWEGLER
Interno - 2017813 - RICARDO EVANDRO MENDES
Interna - 2408296 - SORAYA REGINA SACCO