Banca de DEFESA: RAFAEL LUIZ OLIVO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : RAFAEL LUIZ OLIVO
DATE: 28/07/2021
TIME: 14:00
LOCAL: Webconferência via Google Meet - meet.google.com/khy-zroy-sou
TITLE:

In vitro anthelmintic activity of drugs in Eurytrema coelomaticum control.

KEY WORDS:

eurythrematosis; pancreas; praziquantel; nitazoxanide; histopathology.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Eurytrema coelomaticum are trematodes that parasitize cattle, buffaloes, goats, sheep and camelids, and incidentally, humans and felines. The affected animal usually has subclinical pancreatic disease, but the damage caused to health and production is subjugated. The objective of this work was to evaluate the in vitro anthelmintic activity of Praziquantel and Nitazoxanide against the parasite Eurytrema coelomaticum through the evaluation of motility during 3, 12 and 15 hours of incubation and subsequent histopathological examination of the parasites. Examples of E. coelomaticum were captured from the pancreas of naturally infected cattle, collected from animals slaughtered in a slaughterhouse in the city of Concordia-SC, Brazil. The examples were incubated in different plates with appropriate culture media, of 80 mg/mL praziquantel (PZQ) and 200uM/mL nitazoxanide (NTZ) and a control group (no drugs). Motility was investigated after 3, 12 and 15 h of incubation and after this period histopathological examination of the parasites was performed. In 12h of incubation, all parasites in the NTZ and PZQ groups were immobile or dead and on the control group 82% (5/60) had normal motility after 15h of incubation (p <0.01). By histopathological examination, severe damage in several organs, such as vitellogenic gland, intestine, parenchyma, tegument and testis were observed in both treated groups. Lesions were more pronounced in the NTZ group, except in the intestine. It is concluded that PZQ and NTZ have in vitro anthelmintic action against the parasite Eurytrema coelomaticum, causing significative damage to organs and decreasing parasite motility when compared to the control group.

BANKING MEMBERS:
Externa à Instituição - BIANCA PAOLA SANTAROSA - UFVJM
Externa à Instituição - MARIA FRANCISCA NEVES - UNICAMP
Presidente - 2408296 - SORAYA REGINA SACCO