Banca de DEFESA: ANA CAROLINA SPRICIGO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ANA CAROLINA SPRICIGO
DATE: 27/07/2021
TIME: 13:30
LOCAL: Webconferência via Google Meet - https://meet.google.com/efv-jrug-zev
TITLE:

Genotypic and antimicrobial resistance profile of avian pathogenic Escherichia coli.

KEY WORDS:

birds; chickens; bacterium; epidemiology; susceptibility.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Escherichia coli pathogenic avian (APEC) is responsible for several anatomopathological conditions in poultry, which cause great damage to the poultry sector. Knowing the epidemiology of this bacterium is essential to effectively control this pathogen, therefore, the objective of this work was to evaluate the pathogenicity of Escherichia coli strains isolated from broiler chickens and to perform in vitro tests on strains classified as APEC to identify their capacity for biofilm formation and susceptibility to antimicrobials and sanitizers routinely used in the poultry breeding process. In this study, 60 Escherichia coli isolates from a strain bank of the Animal Health laboratory were used, obtained from clinical cases of colibacillosis in broiler chickens located in the west of Santa Catarina, in 2020. In the real-time Polymerase Chain Reaction test it was identified that 100% of the isolates had the hlyF and ompT genes, 98.3% the iroN and iss genes and 83.33% the iutA gene, being all evaluated strains classified as APEC. In the in vitro evaluations regarding the formation and characterization of biofilms, the APEC samples were characterized as 71.66% weakly forming biofilms and 28.33% did not show bacterial biofilm formation. In the antibiotic susceptibility test, through the disk-diffusion technique, the highest resistance percentages werefound in the molecules of oxytetracycline 33% and doxycycline 31.66%, but about 33%. In the test of sensitivity to sanitizers, the multiresistant APEC strains evaluated were sensitive to disinfectants based on quaternary ammonia + glutaraldehyde and to the compound phenol.

BANKING MEMBERS:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Interno - 1756086 - DIOGENES DEZEN
Externa à Instituição - Danila Soares Caixeta - UFMT