Banca de DEFESA: FRANCISCO ELIAS VENDRUSCOLO

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : FRANCISCO ELIAS VENDRUSCOLO
DATE: 22/07/2021
TIME: 13:30
LOCAL: Webconferência via Google Meet - meet.google.com/xki-tceo-wwn
TITLE:

Investigation of Influenza A Virus circulation in finishing pigs.

KEY WORDS:

respiratory disease, pandemic, nasal swab, nasal wipe.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Currently, the intensive model adopted in the large scale swine production chain predisposes the development of enzootic nature diseases, often involving a complex of etiological agents and causing syndromes that lead to a decrease in productivity rates and frequently an increase in herds mortality rate. The Influenza A virus (IAV) is an etiological agent that is part of the so-called swine respiratory disease complex (SRDC), it can be associated with nonspecific clinical signs such as fever, apathy, cough, prostration, inappetence, weight loss and frequently associated with pneumonia injuries responsible for carcasses condemnation in the slaughterhouse. The possibility of viral rearrangements associated with variants of avian, human and swine influenza increases the importance on a pandemic event, reiterating the importance of monitoring the virus prevalence. The adoption of good production practices (GPP) and adequate biosecurity is described as a way to prevent the virus spreading. Different production models (in size and levels of biosecurity) may be linked to the prevalence of IAV and must be monitored in order to develop effective strategies to reduce the damage in commercial herds. The work was developed in an industry situated in the middle-west of Santa Catarina through a checklist applied to 385 farms in a vertical system of finishing pigs, characterizing them in terms of the biosecurity level and GPP. An amount of 35 farms were submitted to the collection of nasal swabs for the detection of IAV through the polymerase chain reaction technique (RT-PCR), a total of 529 samples will be submitted to the technique. A technique for collecting material for diagnosis through the use of nasal wipes was also used to evaluate the method, totaling 80 samples. After carrying out the checklist on the farms, four groups were characterized according to the levels of biosecurity and GPP adopted: G1 - farms with less than 700 animals and with a low standard of biosecurity (145 farms); G2 - farms with less than 700 animals and a high standard of biosecurity (56 farms); G3 - farms with more than 700 animals and low biosecurity standard (74 farms) and G4 - farms with more than 700 animals and high biosafety standard (110 farms). 30 samples of nasal swabs and nasal wipes have been carried out on a farm for a pilot test, resulting in 86.6% of animals positive for IAV through the traditional collection of nasal swabs and 83.3% of positivity using the technique of nasal wipes. As a result of the collections of the experiment, 15 positive samples for IAV were obtained, being 12 through the collection from traditional nasal swab and 3 through the nasal wipes method. Analysis of variance was performed to compare the methods, showing a statistical difference (p≤0.05) between them, with the nasal swab method showing a lower “ct” value.

BANKING MEMBERS:
Externa à Instituição - FRANCINI KLAUMANN
Interno - 1756086 - DIOGENES DEZEN
Presidente - 586.812.306-97 - JANICE REIS CIACCI ZANELLA - EMBRAPA