Banca de DEFESA: LUCA FRONDANA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LUCA FRONDANA
DATE: 22/07/2021
TIME: 15:00
LOCAL: Webconferência via Google Meet - https://meet.google.com/ywo-uoey-bfd
TITLE:

Determination of bacterial populations in sardine samples condemned for the occurrence of histamine.

KEY WORDS:

metagenomics; canned; fish; biogenic amines, scombrotoxicosis

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Every year hundreds of tons of sardines are discarded due to the presence of histamine, a biogenic amine with the potential to cause poisoning in humans, formed with the biological degradation of the musculature of fish. Although there are several studies in the area, to our knowledge, no work has really demonstrated which bacterial groups are most important for the formation of histamine in sardines. Thus, the experiment aimed to qualitatively and quantitatively characterize the bacterial populations present in sardine samples with different concentrations of histamines. The samples were submitted to Next Generation DNA sequencing (Next Generation Sequencing - NGS) to assess the microbial load. After processing and analyzing the identified sequences, it was possible to conclude that there were significant differences (p<0.05) between histamine and histamine-free samples. Bacteria commonly associated with fish decomposition and with optimal growth related to mesophilia, such as Shewanella ssp. and Photobacterium ssp., had higher relative abundance in samples with histamine when compared to samples without histamine (p<0.05), while psychotropic bacteria not related to spoilage, such as Psychrobacter ssp. and Pseudoalteromonas ssp. had higher relative abundance in samples without histamine (p<0.05). Thus, it is suggested that samples with histamine concentration above 200ppm had a different treatment from those without histamine, probably the cold chain was not correctly applied from the moment of fishing until freezing and sending the fish for processing.

BANKING MEMBERS:
Presidente - 2613308 - DELANO DIAS SCHLEDER
Externo à Instituição - GUSTAVO ADOLFO MARCONCIN FARIA
Externa ao Programa - 1800361 - MARLISE POMPEO CLAUS