Banca de DEFESA: LEONARDO BENASSI DE BORBA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : LEONARDO BENASSI DE BORBA
DATE: 29/12/2020
TIME: 14:00
LOCAL: Web Conferência via xxx - xxxx
TITLE:

Antibiotics for veterinary use: Preliminary assessment of phytotoxicity and impact on the enzymatic activity of soil microorganisms.

KEY WORDS:

antibiotics, animal waste; phytotoxicity; fluorescein; antimicrobial resistance.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Reduction of environmental impacts is one of the main challenges in animal production, since the high population density of animals leads to increased pressure of infection in the facilities and in order to maintain sanity and increase productivity, there is a frequent use of antimicrobials. Thus, in addition to the large production of waste products (i.e., organic matter), the situation is aggravated by the presence of several biologically active drugs in these waste products, which are often used as biofertilizers. Veterinary drugs are considered emerging pollutants, with antibiotics being the most harmful class, as they are biologically active molecules that will cause deleterious effects on phytoproductivity and soil microbiology and predispose to the appearance of superbacterias due to the effect of selective pressure. Soil enzymatic activities are often used to monitor impacts of management, agricultural practices or environmental contamination. One of the techniques used to quantify enzymatic activities is the use of fluorescein diacetate (DAF) hydrolysis, suggested as a possible indicator to measure the global microbial activity of a soil. Therefore, the objective of this work was to evaluate the impact of veterinary antibiotics on phytoproductivity and soil microbiology through the hydrolysis of fluorescein diacetate. The soil was collected in the city of Camboriú - SC and was submitted to hydrolysis analysis of fluorescein diacetate and to the phytotoxicity test with the species Lactuca sativa (lettuce). According to the results, the most appropriate exposure time to assess the impact of antibiotics on soil microbiology was 24 hours, while the incubation time for the analysis of the hydrolysis of DAF that demonstrated the best results was 3 hours. In general, both Amoxicillin and Norfloxacin showed less DAF hydrolysis activity in the soil compared to the control soil. For the dry and wet biomass parameters of lettuce, colistin 2% and ampicilin 1% were the treatments that demonstrated the greatest negative influence on the seedling biomass of lettuce, demonstrating the phytotoxic potential of antibiotics. In this way, the work makes it evident that the use of excessive antibiotics in animal production can lead to environmental impacts and, in the future, to public health problems.

BANKING MEMBERS:
Presidente - 1836822 - CLEDER ALEXANDRE SOMENSI
Externa ao Programa - 1351550 - CRISTIANE VANESSA TAGLIARI CORREA
Externo à Instituição - RENAN CHIPRAUSKI TESTOLIN - UVI