Evaluation of seroconversion induced by commercial inactivated vaccines against Salmonella Enteritidis and Salmonella Typhimurium.
vaccines; intramuscular; salmonellosis; tool.
Inactivated vaccines are intended to reduce contamination and assist in the control of salmonellosis in birds of commercial laying. The objective of the work was to evaluate three commercial inactivated vaccines of Salmonella Enteritidis and Salmonella Typhimurium (Salmonella Se + St), available in the Brazilian market, to induce the immune response, through serology (ELISA), and to detect antibodies directed to serotypes of the group B and D. For this, four treatment groups were analyzed, containing 20 birds of commercial laying, vaccinated at 18 weeks of age. The birds were vaccinated with the Gallimune SE + ST - Boehringer Ingelheim vaccine (dose 0.3 mL / bird for intramuscular route), Salenvac T - MSD vaccine (dose 0.5 mL / bird for intramuscular route), Avisan Secure - Hipra vaccine (dose 0.5 mL / bird for intramuscular route), according to information from the manufacturers and Not vaccinated (Control group). Serum samples were collected at 14, 21, 28, 49, 84, 119 and 161 days after vaccination, and systemic seroconversion was evaluated after vaccination with inactivated vaccines intramuscularly against Salmonella Enteritidis and Salmonella Typhimurium. After the start of the laying, at 25 weeks (peak production), egg samples were collected for analysis of detection and quantification of antibodies transferred from the bird to the yolk. The experiment and the collection of samples were carried out at the Federal University of Minas Gerais and laboratory analyzes were carried out at the Boehringer Ingelheim laboratory in Paulínia. All vaccines analyzed had good seroconversion for groups B and D, as well as the transfer of antibodies to the egg yolks produced by these birds. There was a small difference between the vaccines used, in relation to the persistence of the protective antibody titer during the birds' life span.