Banca de DEFESA: JHONATAN SPERANDIO
Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.STUDENT : JHONATAN SPERANDIO
DATE: 06/08/2020
TIME: 09:00
LOCAL: Web Conferência via Google Meet - Sessão fechada.
TITLE:
Thymus vulgaris essential oil activity against Salmonella enterica serovar Heidelberg isolated from broiler chicken.
KEY WORDS:
Antibacterial activity, thyme, thymol, salmonellosis, sanitizer.
PAGES: 40
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:
Salmonella enterica serovar Heidelberg (SE) is often isolated from samples in broiler chickens’ farms. Its environmental resistance is a factor that harms the entire meat production chain. Altough there is a higher intensification in farms biosecurity procedures, the pressure from the industry to eliminate this pathogen is increasing once salmoneloses can be a problem to the costumers. There are several products with ammonia in its composition that are used for desinfection of poultry shed structures. These products usually have high toxicity to the environment, the animals and people who manipulates it. On the other hand, research on essential oils (EO) and its bacterial activity have been increasing through the years. The objective of this study was to evaluate the in vitro effects of Thymus vulgaris essential oil (TEO), popularly known as thyme, on Salmonella enterica sorovar Heidelberg. Qualitative analysis of the oil revealed thymol (47,3%), p-Cimene (26.8%) and linalool (5.2%) as major compounds. Viable plankton cell quantification technique was used for in vitro antibacterial assays. Four different concentrations of TEO (0.1%, 0.2%, 0.4% and 0.8%) were tested in five strains of SE isolated from broiler chickens farms plus ATCC 8623. In addition, the activity of the monoterpene thymol at concentrations of 0.023%, 0.047%, 0.071% and 0.094% was also evaluated. For the cytotoxicity assay, MTT technique was used in IEC-6 intestinal cells and NCTC fibroblasts in concentrations of 0.0625%, 0.125%, 0.25% and 0.5% of TEO and thymol, in 24h, to find the IC50. RESULTS OMITTED. PATENT APPLICATION IN PROGRESS.
BANKING MEMBERS:
Externa à Instituição - SHIRLEY KUHNEN - UFSC
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Externa à Instituição - ANA PAULA ALMEIDA BASTOS - USP