Banca de DEFESA: MARINARA MACELAI BEZERRA

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MARINARA MACELAI BEZERRA
DATE: 29/07/2020
TIME: 14:00
LOCAL: Web Conferência via Google Meet - meet.google.com/fno-xfkw-wwb.
TITLE:

Serological and molecular detection of Ehrlichia spp. for characterization of strains in naturally infected dogs in the West and Coastal regions of Santa Catarina.

KEY WORDS:

Ehrlichiosis; RIFI; TRP19; TRP36; Hemoparasitosis.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

In order to detect the presence of the Ehrlichia canis and characterize its strain serologically, blood samples were collected from a population of 70 dogs suspected of having hemoparasitosis in the West and Coast of the state of Santa Catarina. The dsb5 gene was used as a target for PCR for diagnostic purposes, and an Indirect Immunofluorescence Reaction (RIFI) for Ehrlichia spp. Was also performed, as well as a specific ELISA for TRP19, TRP36 and TRP32 peptides to assess serological diversity. The prevalence of the disease was 14.29%, with a confidence limit ranging from 7.07% to 24.71%. Of the animals evaluated 3/70 (4.3%) were positive for CRP, all in the western region of SC, and 7/70 (10%) serologically positive in RIFI, two from Concórdia and five from Joinville. Still in relation to serology, the ELISA for TRP19, which detects specific antibodies to E. canis, detected three positive animals (4.3%), two in the West and one in the Coast, the ELISA for the TRP36 peptide genogroup US was also positive for three dogs (4.3%), all from Joinville and the ELISA for the TRP36 Brazilian genogroup was positive in two dogs from the Litoral (2.8%), suggesting the existence of at least two E. canis genogroups in the Litoral do State (US and Brazilian). In Concórdia, positive animals for E. canis did not react to any of the genogroups, indicating the presence of another strain in the region. It is concluded that even with a lower incidence than that reported in the other states of Brazil, the serological knowledge of the circulating strains can help in data on the severity of the disease, possibilities of treatment and even on vaccine development.

BANKING MEMBERS:
Externo à Instituição - HUGO L. R. COSTA - UNESP
Externa à Instituição - MARIA FRANCISCA NEVES - UNESP
Presidente - 2408296 - SORAYA REGINA SACCO