Banca de DEFESA: ALESSANDRA APPEL

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : ALESSANDRA APPEL
DATE: 27/07/2020
TIME: 16:00
LOCAL: Web Conferência via Google Meet - meet.google.com/pec-zsrh-ykn
TITLE:

Disinfection of the new poultry litter (shavings) substrate by the photohydroionization process.

KEY WORDS:

Poultry, biosecurity; PHI e UV; sanitization, litter substrate.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

The procedures implemented in the farms, such as the intensive care of cleaning and disinfection of the facilities and all materials that will have contact with the animals are essential for disease prevention. Therefore, the aim of this study was to test the disinfection of new shavings, used as substrate for litter, through Photohydroionization (PHI). The experiment was carried out with 15 kg of new shaving, sterilized by autoclaving and challenged with pre-established bacterial, fungal and viral inoculum. The bacterial agents selected were Escherichia coli strains, Staphylococcus aureus and Salmonella Abony. The fungal agent used was Saccharomyces cerevisiae, the viral agent was the Gumboro disease virus (IBDV) isolated from the field strain. For each batch, 250 grams of shavings challenged with 32 mL of bacterial and fungal inoculum and 10 mL of viral inoculum were used, being performed 6 repetitions at four different times: 0, 1, 5 and 10 minutes. The disinfection process was performed in a benchtop Photohydroionization equipped with 4 ultraviolet lamps. The agents inoculated in the shavings were recovered after the disinfection process through the analysis of total bacterial and enterobacterial, fungal colonies and viral titer. The data were submitted to the Shapiro Wilk normality test and the regression (REG) and broken line regression (NLIN) analyzes using the SAS. Mean values of reduction were observed, such as, 4,48, 1,73, 1,00 and 0,66 CFU/g of enterobacterial, 3,48, 2,56, 1,70 and 1,77 CFU/g of total bacterial, 3,80; 3,65; 3,51 and 3,38 CFU/g of fungal and 2,84; 2,38, 0 e 0 of viral title per 100 g of shavings at times 0, 1, 5 and 10 minutes, respectively. Enterobacterial and total bacterial counts showed a quadratic effect, while the fungal counts and viruses titer showed a negative linear effect with an increase in the time of the photohydroionization process. The linear response plateau (LRP) effect for enterobacterial (P <0,0147) was observed, with a minimum time point of 5,46 (R) minutes at minimum contamination of 0,66 CFU/g (L) with a reduction of 82,27% in the pre-established inoculum. For the total bacterial LRP effect (P <0,0001) was observed, with a minimum time point of 1,90 (R) minutes in the minimum contamination of 1,74 CFU/g (L) with a reduction of 50,00% in the pre-established inoculum. For the fungal was found LRP effect (P <0,0002), with a minimum time point of 7,93 (R) minutes in the minimum contamination of 3,38 CFU/g (L) with a reduction of 11,00% in the pre-established inoculum. For the viral LRP effect (P <0,0001) was observed, with a minimum time point of 5,01 (R) minutes in the minimum contamination of 0% viral title per 100 g of shavings (L) with a reduction of 64,78% in the pre-established inoculum. Data show a reduction in the count of inoculated agents. This reduction occurs at different times according to the agent in question, partiallyfulfilling the purpose of shaving disinfection.

BANKING MEMBERS:
Externo à Instituição - IVOMAR OLDONI
Externa à Instituição - PRISCILA DINIZ LOPES
Presidente - 1085177 - VANESSA PERIPOLLI