Banca de DEFESA: MAIARA CRISTINE TITON

Uma banca de DEFESA de MESTRADO foi cadastrada pelo programa.
STUDENT : MAIARA CRISTINE TITON
DATE: 07/07/2020
TIME: 08:00
LOCAL: Webconferência pelo Google Meet: https://meet.google.com/kjw-sncc-sne
TITLE:

Optimization of molecular detection assay of Salmonella (MDS^®) for animal health: boot sock sample.

KEY WORDS:

Technology; science; bacterial isolation; poultry.

PAGES: 45
BIG AREA: Ciências Agrárias
AREA: Medicina Veterinária
SUMMARY:

Brazilian beef poultry is prominent in the production and exportation of chicken meat, the detection of Salmonella sp. is important, because it interferes in the consumer market, for being one of the main microorganisms involved in foodborne diseases. In order to control and monitor Salmonella sp. in commercial poultry and slaughter establishments, Normative Instruction Nº 20, of 10/21/2016 (MAPA), requires laboratory testing of boot sock prior to slaughter, carried out in accordance with Ordinance 126 of 11/03/1995 (MAPA). In order to streamline the detection process, this study aimed to optimize the 3M^TM Molecular Detection Assay 2: Salmonella (MDS^®), as per Ordinance 126, for boot sock sample. The development of the methodology followed ISO 16140-2:2016, and 300 naturally contaminated boot sock samples were analyzed, collected from aviaries in western Santa Catarina, and 30 samples artificially contaminated with Salmonella ATCCs in dilutions 10², 10³, 10⁴ for each protocol, reference (Ordinance 126) and alternative (A e B). Of the 300 samples processed in protocol A, 45 positive samples were obtained for Salmonella sp., 242 negative, 1 false positive and 12 false negative, while of the 300 samples analyzed in protocol B, 40 positive, 256 negative, 1 false-positive and 3 false-negative. Resulting for protocol A, a sensitivity of 79%, specificity of 100%, Positive Predictive Value (PPV) of 98% and Negative Predictive Value (NPV) of 95%; and in protocol B, 93% sensitivity, 100% specificity, 98% PPV and 99% NPV were obtained. In addition, the result of Fisher’s exact test in both protocols was p-value < 0,05, demonstrating association with the reference method, concluding that the MDS^® can be used as a screening method.

BANKING MEMBERS:
Presidente - 1989957 - ALESSANDRA FARIAS MILLEZI
Externa à Instituição - ANA PAULA ALMEIDA BASTOS - USP
Interno - 1756086 - DIOGENES DEZEN
Externa à Instituição - Danila Soares Caixeta - UFLA