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CESAR RODRIGO DE SOUZA SURIAN
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Pancreatic profile in bovine euritrematosis according to parasitic burden
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Leader : RICARDO EVANDRO MENDES
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MEMBRES DE LA BANQUE :
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MATHEUS PEDROTTI DE CESARO
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RICARDO EVANDRO MENDES
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RONALDO MICHEL BIANCHI
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Data: 19 juin 2020
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Afficher le Résumé
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Bovine Euritrematosis in Brazil is caused by Eurytrema coelomaticum, a trematode of pancreatic ducts in ruminants, predominantly in the southern states. Macroscopic pancreatic lesions were related to pancreatic atrophy, fibrous interstitial pancreatitis, and total or partial pancreatic duct obliteration. It is conjectured these lesions may cause disorders in the secretory functions of the pancreas, digestive and metabolic processes dependent on them. The aim of this work was to determine if there is a decrease in the digestibility of nutrients, serum, hematological and fecal changes in parasitized cattle correlated with parasite burden. Pancreas and respective blood and fecal samples from 119 bovines, belonging to different breeds and from different rural properties, collected in the municipal slaughterhouse in Concórdia-SC, were used. Stool samples were kept at 4ºC until they were subjected to gelatin digestion tests in a tube and radiographic film digestion. The following biochemical tests were performed: amylase, lipase, glycemia, fructosamine, cholesterol, triglycerides, total proteins, albumin, and globulins. After that, the biochemical analyzes were correlated with the numerical quantity of parasites found in the pancreas (post-mortem). The prevalence of euritrematosis in the region was approximately 60%. There was a greater amount of negative tests in animals with high parasitic load both in the radiographic film digestion test and in the gelatin digestion test (p <0.001), when compared to non-parasitized animals and those with low parasitic load, demonstrating changes in the above mentioned exams only if the number of parasites is significantly high. The activities of the enzymes amylase and lipase were significantly higher in animals with low parasitemia (p <0.05), compared to animals with high parasitic burden and non-parasitized. Also, animals highly parasitized by E. coelomaticum demonstrated hyperproteinemia due to hyperglobulinemia (p <0.05), correlating this with the inflammatory response with the formation of antibodies. It is concluded that in cases of high parasitemia, there is a significant change in the tests of digestion in feces and that in cases of low parasitemia there are significant changes in the values of serum amylase and lipase, both proving biochemical changes in the pancreatic profile, according to parasitemia.
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2
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MAIARA CRISTINE TITON
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Optimization of molecular detection assay of Salmonella (MDS®) for animal health: boot sock sample.
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Leader : ALESSANDRA FARIAS MILLEZI
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MEMBRES DE LA BANQUE :
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ALESSANDRA FARIAS MILLEZI
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DIOGENES DEZEN
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DANILA SOARES CAIXETA
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Data: 7 juil. 2020
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Afficher le Résumé
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Brazilian beef poultry is prominent in the production and exportation of chicken meat, the detection of Salmonella sp. is important, because it interferes in the consumer market, for being one of the main microorganisms involved in foodborne diseases. In order to control and monitor Salmonella sp. in commercial poultry and slaughter establishments, Normative Instruction Nº 20, of 10/21/2016 (MAPA), requires laboratory testing of boot sock prior to slaughter, carried out in accordance with Ordinance 126 of 11/03/1995 (MAPA). In order to streamline the detection process, this study aimed to optimize the 3MTM Molecular Detection Assay 2: Salmonella (MDS®), as per Ordinance 126, for boot sock sample. The development of the methodology followed ISO 16140-2:2016, and 300 naturally contaminated boot sock samples were analyzed, collected from aviaries in western Santa Catarina, and 30 samples artificially contaminated with Salmonella ATCCs in dilutions 102, 103, 104 for each protocol, reference (Ordinance 126) and alternative (A e B). Of the 300 samples processed in protocol A, 45 positive samples were obtained for Salmonella sp., 242 negative, 1 false positive and 12 false negative, while of the 300 samples analyzed in protocol B, 40 positive, 256 negative, 1 false-positive and 3 false-negative. Resulting for protocol A, a sensitivity of 79%, specificity of 100%, Positive Predictive Value (PPV) of 98% and Negative Predictive Value (NPV) of 95%; and in protocol B, 93% sensitivity, 100% specificity, 98% PPV and 99% NPV were obtained. In addition, the result of Fisher’s exact test in both protocols was p-value < 0,05, demonstrating association with the reference method, concluding that the MDS® can be used as a screening method.
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3
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MÁRCIA TONETTO
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Influence of corn particle size and feed physical form on zootechnical performance, digestive tract morphometry and nutrient digestibility in broilers.
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Leader : FABIANA MOREIRA
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MEMBRES DE LA BANQUE :
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FABIANA MOREIRA
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SEBASTIÃO A. BORGES
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VINICIUS GONSALES SCHRAMM
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Data: 10 juil. 2020
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Afficher le Résumé
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Nutrition is one of the most important factors in poultry production, either by cost or animal response to feed quality. The objective of this study trial was to verify the influence of the geometric mean diameter (GMD) of the corn particle and the physical form of the diet on zootechnical performance, digestive tract morphometry and digestibility of feed nutrients in broilers. Were evaluated 3600 male ross 91 broilers from 14 to 39 days of age, distributed according to a completely randomized design in a 2 X 4 factorial scheme, two physical forms of feed (mashed and pelletized) with four different corn granulometry (GMD1: 819-824; GMD2: 1001-1084; GMD3: 1154-1248; GMD4: 1250-1312). All data were submitted to the Shapiro Wilk test and later ANOVA with a 95% confidence level (P>0.05). Pelletized diets improved weight gain WG (2.37 ± 5.25 kg) and feed conversion rate FCR (1.593 ± 0.002) of birds. From GMD2, regardless of the physical form of the diet, the animals presented better results of GP and AC (P<0.05). The weight of the gizzard, depth of ileum crypt and the ratio of villi height and crypt depth were better in birds fed with mashed feed and with GMD2 interval (P<0.05). Regarding the height of the villus of the ileum there was a difference of 17.04 mm in birds fed a diet of the pelletized diet, and analyzing only GMD, the GMD3 interval stood out as being better. No influence the sinze of the intestine neither on nutrient digestibility was observed, regardless of the treatment that the broilers were submitted. Thus, birds fed pelletized diets obtained better zootechnical performance, and corn granulometry from GMD2, presented higher GP and better FRC, regardless of physical f of the feed. However, the diets with GMD2 showed better results for digestive tract morphometry of broilers.
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4
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MARINARA MACELAI LEITE
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Serological and molecular detection of Ehrlichia spp. for characterization of strains in naturally infected dogs in the West and Coastal regions of Santa Catarina.
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Leader : SORAYA REGINA SACCO
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MEMBRES DE LA BANQUE :
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HUGO L. R. COSTA
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MARIA FRANCISCA NEVES
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SORAYA REGINA SACCO
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Data: 10 juil. 2020
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In order to detect the presence of the Ehrlichia canis and characterize its strain serologically, blood samples were collected from a population of 70 dogs suspected of having hemoparasitosis in the West and Coast of the state of Santa Catarina. The dsb5 gene was used as a target for PCR for diagnostic purposes, and an Indirect Immunofluorescence Reaction (RIFI) for Ehrlichia spp. Was also performed, as well as a specific ELISA for TRP19, TRP36 and TRP32 peptides to assess serological diversity. The prevalence of the disease was 14.29%, with a confidence limit ranging from 7.07% to 24.71%. Of the animals evaluated 3/70 (4.3%) were positive for CRP, all in the western region of SC, and 7/70 (10%) serologically positive in RIFI, two from Concórdia and five from Joinville. Still in relation to serology, the ELISA for TRP19, which detects specific antibodies to E. canis, detected three positive animals (4.3%), two in the West and one in the Coast, the ELISA for the TRP36 peptide genogroup US was also positive for three dogs (4.3%), all from Joinville and the ELISA for the TRP36 Brazilian genogroup was positive in two dogs from the Litoral (2.8%), suggesting the existence of at least two E. canis genogroups in the Litoral do State (US and Brazilian). In Concórdia, positive animals for E. canis did not react to any of the genogroups, indicating the presence of another strain in the region. It is concluded that even with a lower incidence than that reported in the other states of Brazil, the serological knowledge of the circulating strains can help in data on the severity of the disease, possibilities of treatment and even on vaccine development.
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5
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DIMITRY MACEDONIO FRACARO BALDISSERA
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Citrate/creatinine ratio “cut-off” reference range in isolated urin samples of healthy dogs.
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Leader : SORAYA REGINA SACCO
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MEMBRES DE LA BANQUE :
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MARIA FRANCISCA NEVES
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MARIO LETTIERI TEIXEIRA
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SORAYA REGINA SACCO
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Data: 17 juil. 2020
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Afficher le Résumé
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The citrate / creatinine ratio serves as a marker of hypocitraturia in the diagnostic aid of urolithiasis in humans. The urolithiasis is an important disease of the domestic canine urinary system, the vast majority of them being composed of calcium oxalate, factors such as the decrease in the concentration of urinary crystallization inhibitors, progressively increase the risk of formation of uroliths, being of great relevance in the diagnostic aid the use of laboratory tests to evaluate cases of urolithiasis, since it is observed relatively frequently and is often relapsing. The purpose of this study was to determine the reference range of the citrate / creatinine ratio in random urine samples in healthy dogs. A total of 46 samples of isolated urine were collected by cystocentesis, catheterization or spontaneous urination of dogs attended at the Center for Clinical and Surgical Practices of the IFC- Campus Concórdia. For this, the animals were divided into groups according to gender, Group I (males n = 17), Group II (females n = 29) and by age group, Group III (adults n = 26) and Group IV (senile n = 20). The male dogs had medians of 12 mg/dL for urinary citrate), urinary creatinine of 64 mg/dL and citrate / creatinine ratio of 0.13; the female dogs 18 mg/dL, 82.3 mg/dL and 0.27, the adults 14.35 mg/dL, 69.10 mg/dL and 0.22 and the senile 15.2 mg/dL, 80.85 mg/dL and 0.13, respectively. To assess the correlation between the age of the animals and the parameters evaluated, Spearman's correlation test was performed (non-normal data). No correlation was found for urinary citrate (r=0.03; p=0.83), urinary creatinine (r=0.16; p=0.26), nor for the urinary citrate / creatinine ratio (r=0.11; p=0.44). However, the values of the citrate / creatinine ratios did not suffer significant variations when compared between the groups, indicating a possible single reference interval for dogs, as occurs with this parameter in human medicine.
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6
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ANDRÉ MAURICIO BUZATO
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Tolfenamic acid in gilts: positive effects on litters performance.
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Leader : JALUSA DEON KICH
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MEMBRES DE LA BANQUE :
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JALUSA DEON KICH
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DIOGENES DEZEN
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PAULO EDUARDO BENNEMANN
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Data: 23 juil. 2020
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Afficher le Résumé
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Postpartum dysgalaxia syndrome (PPDS) is a common and important disorder that affects a swine female, mainly in intensive production systems. PPDS is characterized by hypogalactia in the puerperium; its symptoms may vary as fever, appetite reduction, mastitis in the female and signs of hunger in the piglets. In the majority of the cases, hypogalaxia is not clearly identified, assuming a sub-clinical aspect. Throughout the last decades, in many countries, tolfenamic acid, a nonsteroidal anti-inflammatory drug (NSAID), has been successfully administered to several animal species, as cats, dogs, cattle, and pigs. Tolfenamic acid is recognized for its analgesic, antipyretic and anti-inflammatory properties. The objective of this study was to evaluate the effect of a non-steroidal anti-inflammatory drug (NSAID), based on tolfenamic acid, as a prophylactic treatment of the syndrome of PPDS, on the zootechnical performance of the litters. Gilts (n = 332) were selected and randomly distributed in two treatments groups, n = 157 in treated group and n = 162 in the control group. Thirteen gilts were excluded during select process. The treated group received a single intramuscular injection (1 mL/20 kg tolfenamic acid/ Toldefine CS™) post-farrowing, the control group did not receive any treatment. All piglets (4,466) were weighed at first, fourth and eighteenth day of life. All litter (2,198 and 2,268 piglets for treated and control groups, respectively) were evaluated for weight gain, diarrhea occurrence, piglets and mortality between 4 and 18 days of life. Data were analyzed using the SAS program (2012), differences were considered significant at level of 5% (P <0.05). Piglet´s weight averages were analyzed by covariance considering the effect of the initial weight and the presence of diarrhea. Categorical variables were analyzed by logistic regression. The treated group had 0.41% less mortality until the 18th day of life (p = 0.0285). This rate increases to 4.5% (4.3% and 8.8% for the treated and control group, respectively) among the litters not affected by diarrhea (p = 0.0005). Gilts presenting rectal temperature above 39.5°C or mucopurulent vulvar discharge were characterized with (PPDS). Overall, the weight average at 18 days in piglets without diarrhea was higher, 4.949g, compared to 4.593g, than piglets with diarrhea. Specifically for litters without diarrhea, the total of weight gain in the treated group was 9.0% (p <0.05) greater than the control group. Tolfenamic acid administered to gilts reduced the piglets mortality during the suckling phase and increased weight gain in litters not affected by diarrhea. The pain and discomfort reduction and in the first days of lactation, contribute to a better litters performance.
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7
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ALESSANDRA APPEL
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Disinfection of the new poultry litter (shavings) substrate by the photohydroionization process.
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Leader : VANESSA PERIPOLLI
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MEMBRES DE LA BANQUE :
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IVOMAR OLDONI
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PRISCILA DINIZ LOPES
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VANESSA PERIPOLLI
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Data: 27 juil. 2020
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The procedures implemented in the farms, such as the intensive care of cleaning and disinfection of the facilities and all materials that will have contact with the animals are essential for disease prevention. Therefore, the aim of this study was to test the disinfection of new shavings, used as substrate for litter, through Photohydroionization (PHI). The experiment was carried out with 15 kg of new shaving, sterilized by autoclaving and challenged with pre-established bacterial, fungal and viral inoculum. The bacterial agents selected were Escherichia coli strains, Staphylococcus aureus and Salmonella Abony. The fungal agent used was Saccharomyces cerevisiae, the viral agent was the Gumboro disease virus (IBDV) isolated from the field strain. For each batch, 250 grams of shavings challenged with 32 mL of bacterial and fungal inoculum and 10 mL of viral inoculum were used, being performed 6 repetitions at four different times: 0, 1, 5 and 10 minutes. The disinfection process was performed in a benchtop Photohydroionization equipped with 4 ultraviolet lamps. The agents inoculated in the shavings were recovered after the disinfection process through the analysis of total bacterial and enterobacterial, fungal colonies and viral titer. The data were submitted to the Shapiro Wilk normality test and the regression (REG) and broken line regression (NLIN) analyzes using the SAS. Mean values of reduction were observed, such as, 4,48, 1,73, 1,00 and 0,66 CFU/g of enterobacterial, 3,48, 2,56, 1,70 and 1,77 CFU/g of total bacterial, 3,80; 3,65; 3,51 and 3,38 CFU/g of fungal and 2,84; 2,38, 0 e 0 of viral title per 100 g of shavings at times 0, 1, 5 and 10 minutes, respectively. Enterobacterial and total bacterial counts showed a quadratic effect, while the fungal counts and viruses titer showed a negative linear effect with an increase in the time of the photohydroionization process. The linear response plateau (LRP) effect for enterobacterial (P <0,0147) was observed, with a minimum time point of 5,46 (R) minutes at minimum contamination of 0,66 CFU/g (L) with a reduction of 82,27% in the pre-established inoculum. For the total bacterial LRP effect (P <0,0001) was observed, with a minimum time point of 1,90 (R) minutes in the minimum contamination of 1,74 CFU/g (L) with a reduction of 50,00% in the pre-established inoculum. For the fungal was found LRP effect (P <0,0002), with a minimum time point of 7,93 (R) minutes in the minimum contamination of 3,38 CFU/g (L) with a reduction of 11,00% in the pre-established inoculum. For the viral LRP effect (P <0,0001) was observed, with a minimum time point of 5,01 (R) minutes in the minimum contamination of 0% viral title per 100 g of shavings (L) with a reduction of 64,78% in the pre-established inoculum. Data show a reduction in the count of inoculated agents. This reduction occurs at different times according to the agent in question, partiallyfulfilling the purpose of shaving disinfection.
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8
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MAIARA PETRI VILVERT
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Taurine supplementation in diets for juvenile Nile tilápia (Oreochromis niloticus).
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Leader : ADOLFO JATOBA MEDEIROS BEZERRA
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MEMBRES DE LA BANQUE :
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ADOLFO JATOBA MEDEIROS BEZERRA
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BRUNO CORRÊA DA SILVA
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GABRIEL FERNANDES ALVES JESUS
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Data: 28 juil. 2020
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Taurine is considered an essential conditionally amino acid for fish and their biosynthesis, from methionine and cystine differs between different species of fish and is also affected by the presence or not in the ingredients used in the manufacture of feed, so its supplementation can improve feed efficiency. The present study evaluated the supplementation of taurine in the zootechnical performance, hematological and immunological parameters and in the survival of juveniles of Nile tilapia (Oreochromis niloticus) grown in two types of system, RAS and bioflocs. A control diet with 360 g / kg of crude protein was prepared without fish meal and without taurine supplementation (Control). From the control diet, another diet supplemented with 9.7 g of taurine per kg of feed was produced (Taurine). In cultivation system RAS, the fish were fed manually, five times a day, seven days a week. The tilapia (n = 200, with an average weight of 13.3 g) were distributed in 8 polyethylene boxes of 800 L each, 25 fish per box, in a completely randomized design with 2 treatments and 4 repetitions, lasting eight weeks. In the biofloc system, the tilapia (N = 150, with an average weight of 18.3 g) were distributed in 6 polyethylene boxes of 250 L each, 25 fish per box, in a completely randomized design with 2 treatments and 3 repetitions, with duration of four weeks. The animals were fed manually, three times a day, seven days a week. In both crops, biometrics were performed weekly to adjust food management. The taurine treatment obtained less daily average weight gain and final average weight in relation to the control (p <0.05), however, in the biofloc system the fish that received the taurine supplementation obtained a higher hepatosomatic index (p <0.05 ). It was observed that there was no influence of taurine on the fish condition factor and blood parameters, but there was an increase in the VCM index and a higher N retention in the control treatment fish reared in the RAS (p <0.05). It is concluded that, regardless of the cultivation system, juveniles of Nile tilapia do not benefit from taurine supplementation even when fed diets containing protein-based plant sources.
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9
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DUSTIN ANDRÉ CHAVES HOFFMANN
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Metabolic profile of dairy cows in the prepartum period and their influence on the immediate postpartum period, colostrum quality and newborn immunity.
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Leader : ELIZABETH SCHWEGLER
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MEMBRES DE LA BANQUE :
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DEISE DALAZEN CASTAGNARA
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CARLOS EDUARDO NOGUEIRA MARTINS
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ELIZABETH SCHWEGLER
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Data: 3 août 2020
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The aim of this study was to evaluate which biochemical markers in the pre-delivery of the dairy cow, influenced its immediate post-delivery, the quality of colostrum and the heifer's immunity. It was carried out in a commercial dairy farm in Palma Sola - SC, from July to September 2019, with 52 pregnant multiparous Holstein cows being pre-selected, and those that gave birth to twins or males were discarded from the experiment, leaving 21 cows and 21 calves . All animals were kept on the Compost Barn system receiving the same diet. On days -20 of the expected date of delivery and 24 hours after delivery, blood samples, body condition score (ECC) and animal weighing were performed. If there was no anticipation of delivery, it was collected on day -5. The urine collection of the cows was done in the pre-calving period to measure the pH, and colostrum was collected immediately after calving to determine the brix® percentage. Blood collections from the calves were performed 24 hours after colostration. The time it took the calves to receive colostrum was considered. Calf plasma was used to estimate the efficiency of colostrum and immunity by% brix and total plasma proteins (PPT). In serological samples of cows, calcium (Ca), magnesium (Mg), non-esterified fatty acids (NEFA), beta hydroxy butyrate (BHBA), paraoxonase (PON-1) and albumin were evaluated. In serological samples from calves, PON1, albumin, gamma glutamyl transferase (GGT), Ca and Mg were evaluated. The data on the cow's pre and postpartum metabolic variables, as well as the calf's metabolic variables, and those related to colostrum quality, were subjected to correlation, regression, variance, main components and logistic regression analyzes using the SAS program. In the principal component analysis, NEFA in postpartum was shown to be higher in cows with higher urinary pH in the prepartum period and higher BHBA in the postpartum period. NEFA and BHBA in the postpartum period was higher in cows with lower Ca in the prepartum period. And the cows with the highest NEFA and BHBA in the pre and postpartum were those that had the lowest% brix of colostrum. The% brix of the mother's colostrum directly influenced the% brix and PPT of the heifer. The NEFA in the pre-calving of the cow, had a negative influence on the immunity markers, the higher the NEFA, the lower the% brix of the mother's colostrum,% brix of the heifer and PPT. In multivariate regression analysis, it was shown that pre-delivery NEFA was the marker that most influenced cow postpartum markers and calf% brix, along with colostrum time and colostrum% brix (P = 0.0092 ; r2 = 0.83). Cows with values above the median (≥ 0.08 mmol / L) and quartile (≥ 0.18 mmol / L) of NEFA in the prepartum period, had lower calcemia in the immediate postpartum period (7.16 ± 0.24 mg / dl, P = 0.04; 6.86 ± 0.35 mg / dl, P = 0.01). From the data obtained in our study, we concluded that the prepartum NEFA was the marker that most influenced the immediate postpartum of the cow, being directly related to the calcemia, and also in the immunity of the heifer.
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10
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MAYARA LAIZ MINOTTO MATTEI
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Detection of E. coli as factors of virulence in isolated liver strains and cellulite in poultry injuries.
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Leader : DIOGENES DEZEN
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MEMBRES DE LA BANQUE :
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ALESSANDRA FARIAS MILLEZI
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DIOGENES DEZEN
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SABRINA CASTILHO DUARTE
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Data: 4 août 2020
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The avian cellulite is characterized by the formation of subcutaneous fibrinonecrotic plaques and by the inflammation of the skin, leading to serious economic loss due to partial slaughter sentences. Escherichia coli (APEC) is the principal isolated agent in the cases of cellulite. The APEC´s are classified as Extraintestinal Pathogenic E. coli (ExPEC) and show similarities with humans strains, suggesting a zoonotic potential. With the aim of characterizing the agent involved in the cases of the avian cellulite, was performed the bacterial isolation and the molecular detection of this virulence factors (iss e felA), in 130 carcasses with cellulite injuries coming from a slaughterhouse in the west of Santa Catarina. E. coli strains were isolated from the cellulites injuries and livers by means of screening and identified through biochemical tests, the agent was detected in 62,3% of carcasses, being 52.3% from the cellulitis, 55,3% of livers and 46,1% in both samples. From positive samples, were gotten 253 strains of E. coli, which were screened for the genes of serum resistance (iss) and responsible for adhesion (felA) by means of PCR. The percentage of positivity of the iss gene on the skin and liver were 73, 6% and 85,6% respectively. The frequency of the felA gene in the skin were of 27,3% and in the liver of 13,6%. Most of the isolated strains showed at least one of the virulence factors, and in 17,4% both genes were detected. The isolation of the agent and the frequency found of genes, indicate that the E. coli strains coming from cellulite injuries were possibly causing factors of septicemia and an important source of food contamination, suggesting the review of the sentencing criteria, reducing the potential risks to public health.
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11
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LEANDRO FIGUEIRA SELAU
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Evaluation of seroconversion induced by commercial inactivated vaccines against Salmonella Enteritidis and Salmonella Typhimurium.
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Leader : BRENO CASTELLO BRANCO BEIRÃO
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MEMBRES DE LA BANQUE :
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LUIZ FELIPE PINHO PEREIRA
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ANTONIO CARLOS PEDROSO
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BRENO CASTELLO BRANCO BEIRÃO
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Data: 5 août 2020
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Inactivated vaccines are intended to reduce contamination and assist in the control of salmonellosis in birds of commercial laying. The objective of the work was to evaluate three commercial inactivated vaccines of Salmonella Enteritidis and Salmonella Typhimurium (Salmonella Se + St), available in the Brazilian market, to induce the immune response, through serology (ELISA), and to detect antibodies directed to serotypes of the group B and D. For this, four treatment groups were analyzed, containing 20 birds of commercial laying, vaccinated at 18 weeks of age. The birds were vaccinated with the Gallimune SE + ST - Boehringer Ingelheim vaccine (dose 0.3 mL / bird for intramuscular route), Salenvac T - MSD vaccine (dose 0.5 mL / bird for intramuscular route), Avisan Secure - Hipra vaccine (dose 0.5 mL / bird for intramuscular route), according to information from the manufacturers and Not vaccinated (Control group). Serum samples were collected at 14, 21, 28, 49, 84, 119 and 161 days after vaccination, and systemic seroconversion was evaluated after vaccination with inactivated vaccines intramuscularly against Salmonella Enteritidis and Salmonella Typhimurium. After the start of the laying, at 25 weeks (peak production), egg samples were collected for analysis of detection and quantification of antibodies transferred from the bird to the yolk. The experiment and the collection of samples were carried out at the Federal University of Minas Gerais and laboratory analyzes were carried out at the Boehringer Ingelheim laboratory in Paulínia. All vaccines analyzed had good seroconversion for groups B and D, as well as the transfer of antibodies to the egg yolks produced by these birds. There was a small difference between the vaccines used, in relation to the persistence of the protective antibody titer during the birds' life span.
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12
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GABRIELA TONIAL KNAPPMANN BITTENCOURT
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Influence on the serological response of birds to vaccination against Infectious Bronchitis, in broiler flocks positive for Mycoplasma Sinoviae.
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Leader : PAULO AUGUSTO ESTEVES
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MEMBRES DE LA BANQUE :
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PAULO AUGUSTO ESTEVES
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DIOGENES DEZEN
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ARLEI COLDEBELLA
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Data: 5 août 2020
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Afficher le Résumé
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Especially in recent years, Brazilian poultry has achieved high growth rates, becoming the third major worldwide producer of chicken meat and leader in exports, reaching 142 countries. Factors such as animal health and welfare, quality and price have contributed to improving productivity in the sector. Given this, the concern with the health of animals is one of the main challenges of the market, and the control of Infectious Bronchitis of chickens (IB) and the eradication of Mycoplasma synoviae (Ms) in breeding farms are issues of enormous animal health relevance. IB, caused by a coronavirus, the Infectious Bronchitis Virus (IBV), is an acute and highly contagious viral disease. Mycoplasmosis, on the other hand, is caused by the smallest existing bacteria, Mycoplasma synoviae (Ms), has great capacity for dissemination in the breeding flock, and once the flock is positive, it remains with this status until the end of its life. Both diseases show similar clinical signs affecting the respiratory part, becoming secondary to each other. In the present study, the vaccine titers of Infectious Bronchitis were evaluated in order to verify the existence of interference in the serological response against IBV by the positivity of Ms. For this, the results of 326 serum samples collected in the period from 2014 to 2018 in batches of matrices of an agribusiness in the West of Santa Catarina were verified. To evaluate the effect of the presence of Mycoplasma Sinoviae on the mean titer of bronchitis, the t test was applied in order to compare unpaired data. Through the present analysis it was verified there were no interference of Ms in the antibody titers against the Infectious Bronchitis virus (IBV).
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13
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DANIEL FERNANDO RODIGHERI
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Risk factors associated with locomotor problems in broilers.
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Leader : PAULO AUGUSTO ESTEVES
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MEMBRES DE LA BANQUE :
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ARLEI COLDEBELLA
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DIOGENES DEZEN
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PAULO AUGUSTO ESTEVES
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Data: 6 août 2020
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Afficher le Résumé
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Broiler mortality is a valuable indicator that must be managed in detail. Among the factors of mortality of birds, the object of the present study was the elimination by locomotor problems that affects the majority of poultry populations. In order to find out which zootechnical and environmental factors had the more important impact on the number of birds eliminated by locomotor problems during the life of 139 flocks (3,445,583 birds) raised in an intensive production system under field conditions. The data were previously examined and subsequently submitted to a logistic regression analysis using the NLMIXED procedure of the “Statistical Analysis System ©” (SAS, 2012), to determinate the significance of each factor in relation to the mortality due to locomotor problems. The length of the break, the feed intake in the pre-initial phase, the weight ratio between 14 and 7 days of age and the age of slaughtering, are quantitative variables that presented a level of significance over 95% being considered influential causes for locomotor problems. The classification of producers in the zootechnical result and the presence of environmental enrichment were categorical variables that presented as causes of elimination due to locomotion problems with a significance level above 95%. We conclude that it is possible to associate locomotor problems in broilers with the consumption of the pre-initial diet and its impact on the intermediate weights at the age of 7 and 14 days as well as management factors of birds that may generate skeletal overload.
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14
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JHONATAN SPERANDIO
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Thymus vulgaris essential oil activity against Salmonella enterica serovar Heidelberg isolated from broiler chicken.
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Leader : ALESSANDRA FARIAS MILLEZI
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MEMBRES DE LA BANQUE :
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ALESSANDRA FARIAS MILLEZI
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ANA PAULA ALMEIDA BASTOS
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SHIRLEY KUHNEN
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Data: 6 août 2020
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Afficher le Résumé
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Salmonella enterica serovar Heidelberg (SE) is often isolated from samples in broiler chickens’ farms. Its environmental resistance is a factor that harms the entire meat production chain. Altough there is a higher intensification in farms biosecurity procedures, the pressure from the industry to eliminate this pathogen is increasing once salmoneloses can be a problem to the costumers. There are several products with ammonia in its composition that are used for desinfection of poultry shed structures. These products usually have high toxicity to the environment, the animals and people who manipulates it. On the other hand, research on essential oils (EO) and its bacterial activity have been increasing through the years. The objective of this study was to evaluate the in vitro effects of Thymus vulgaris essential oil (TEO), popularly known as thyme, on Salmonella enterica sorovar Heidelberg. Qualitative analysis of the oil revealed thymol (47,3%), p-Cimene (26.8%) and linalool (5.2%) as major compounds. Viable plankton cell quantification technique was used for in vitro antibacterial assays. Four different concentrations of TEO (0.1%, 0.2%, 0.4% and 0.8%) were tested in five strains of SE isolated from broiler chickens farms plus ATCC 8623. In addition, the activity of the monoterpene thymol at concentrations of 0.023%, 0.047%, 0.071% and 0.094% was also evaluated. For the cytotoxicity assay, MTT technique was used in IEC-6 intestinal cells and NCTC fibroblasts in concentrations of 0.0625%, 0.125%, 0.25% and 0.5% of TEO and thymol, in 24h, to find the IC50. RESULTS OMITTED. PATENT APPLICATION IN PROGRESS.
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15
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MARCELO FELIPE GÜTHS
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Alternatives to the use of antibiotics in pig production.
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Leader : IVAN BIANCHI
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MEMBRES DE LA BANQUE :
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JOSÉ CRISTANI
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IVAN BIANCHI
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PAULO EDUARDO BENNEMANN
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Data: 6 août 2020
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Afficher le Résumé
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The objective of this work was to evaluate the impact of substitution of antibiotics used in pig feed with alternative additives in nursery, growing and finishing pigs. The experiment initially used 1,091 weaned pigs divided into 38 pens with six treatments during nursery and in the growing and finishing stage, 850 animals remained. At weaning the animals were divided by sex (females and males), submitted to the vaccination protocol and weighed individually to achieve the homogeneous distribution among the 6 treatments, being T1: antibiotic free feed, T2: antibiotic feed, T3: prebiotic feed, T4: probiotic feed, T5: essential oil feed, T6: organic acid feed. There was no difference in conversion rate between treatments at the nursey (P = 0.1969) or in growing and finishing stage (P = 0.8225). There was also no difference in the average daily weight gain in nursey (P = 0.05) and in growing and finishing stage (P = 0.3404). The cost of injectable interventions did not differ between treatments or at the nursey (P = 0.9804) or in growing and finishing stage (P = 0.3102). The pneumonia index in all treatments was above 1.0, indicating a respiratory challenge. The substitution of prophylactic use of antibiotics in feed in pig production is possible and the use of alternatives has not provided benefits.
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16
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LUCIANO BRANDALISE
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Acclimation of Mycoplasma hyopneumoniae-negative replacement gilts naturally exposed to the agent.
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Leader : DIOGENES DEZEN
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MEMBRES DE LA BANQUE :
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DAVID EMILIO SANTOS NEVES DE BARCELLOS
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DIOGENES DEZEN
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FABIANA MOREIRA
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Data: 6 août 2020
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Afficher le Résumé
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Negative Mycoplasma hyopneumoniae (M. hyopneumoniae) replacement gilts provide greater health security to breeding stock. However, in M. hyopneumoniae positive herds, the introduction of gilts presents a higher risk for the vertical transmission at first farrowing, because the transmission of the agent is slow and excretion occurs for an extended period. This study aimed to evaluate the infection dynamics of M. hyopneumoniae in negative replacement gilts, introduced in endemically positive Brazilian farms. For this, ninety-eight gilts, negative for M. hyopneumoniae, were housed in three commercial farms positive for the agent. The animals were arranged in collective pens, which allowed contact with the pens of gilts previously housed on the farm, for a period of 21 days. The infection dynamics was evaluated by detection of M. hyopneumoniae by qPCR and by detection of antibodies by ELISA, from samples of laryngeal swab and blood, respectively. Samples were collected at the time of accommodation (150 days old; do), 165, 180, 210, 240, 270, 300 do and pre-farrowing. It was observed that the transmission occurred quickly in farms A and B, being necessary 25,2 and 23,9 days for 95% of the gilts to be positive in the qPCR. There was no influence on the number of seeders at the time of exposure, but their absence (farm C) extended the transmission period to 69,4 days. Were necessary an average of 162.2 days after the first detection by qPCR so that 85% of the females stop excreting the agent. . There was no significant effect of the farm (p> 0.05) on the infection dynamics, showing an intrinsic characteristic of the agent. The serology results were similar to the herd infection curve, however, as expected, the detection of antibodies occurred after the detection of the agent. At the time of pre-farrowing, 100% of the seroconverted gilts and 36.7% of the gilts remained positive in the qPCR. For evaluation of possible sow-piglet transmission, near weaning nasal swab were collected 75 piglets from 15 litters from negative sows and 75 piglets from 15 litters from positive sows in the qPCR in the pre-farrowing. Only 1.33% of the piglets were positive at weaning, being offspring of females positive in qPCR in pre-weaning and at weaning, both from the same farm. However, it was not possible to detect significant differences (p> 0.05) for this variable. In the conditions of this study, acclimatization by natural exposure to M. hyopneumoniae proved to be efficient, and that possibly other factors (bacterial load, strain-specific immunity, strain virulence, etc ...) may limit the transmission of the female agent to her litter and thus mitigate the onset of the disease in the field.
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17
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ANDRESSA VIEIRA DE MORAES
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Microencapsulated probiotics in Oreochromis niloticus fingerling.
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Leader : ADOLFO JATOBA MEDEIROS BEZERRA
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MEMBRES DE LA BANQUE :
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ADOLFO JATOBA MEDEIROS BEZERRA
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BRUNO CORRÊA DA SILVA
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DÉBORA MACHADO FRACALOSSI
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Data: 7 août 2020
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Afficher le Résumé
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Microencapsulation is a biotechnology that allows the viability of probiotics in the industrial process, being a new technique and still little explored. The objective of this work was to analyze individually the effect of two microencapsulated pro biotic additives Add Life Pro Sacch Acqua (S. cerevisiae), and Add Life Pro Bacil Acqua (Bacillus spp) on the diet of O. niloticus fingerlings about microbiological viability in the diet after extrusion process, changes in the microbiota of the host, blood immunological profile of fish, zootechnical parameters and survival of these against the experimental challenge with A. hidrophyla. For this, 630 fingerlings of the species were distributed in nine experimental units (UE), 70 fish each, divided into three treatments (Control, SACCH and BACIL), in triplicate, and fed for 45 days. The microbiological count of the diet was performed after extrusion, on the first day of the experimente, and every 15 days until its completion. After this period, five fish per E U were assigned to hematological evaluations, five for immunological analysis, five for intestinal tract microbiota study, and five for collection of fecal material for intestinal microbiota metagenomic analysis. And 20 animals per treatment were directed to the experimental challenge for 11 days. All individuals were used to calculate zootechnical indices. As preliminary results, the treatments BACIL and SACCH obtained higher survival during the cultivation time (12%), lower feed conversion and higher spec ific growth rate when compared to the control. These data demonstrate that microbiological viability remained after diet extrusion, and brought zootechnical benefits to the host after supplementation.
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18
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FRANCINE OTILIA VOGEL
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Application for estimating the weight of beef cattle.
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Leader : CARLOS EDUARDO NOGUEIRA MARTINS
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MEMBRES DE LA BANQUE :
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CARLOS EDUARDO NOGUEIRA MARTINS
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DIEGO PERES NETTO
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FERNANDO JOSE BRAZ
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Data: 7 août 2020
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Afficher le Résumé
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The present work developed an application to estimate the body weight of beef cattle. The project evaluated 91 animals from three feedlots, all animals were male of Taurine breeds and their crosses, with an average weight ranging between 255 and 506 kg. The animals were weighed on an electronic scale without previous fasting. The measurement of the thoracic perimeter was performed caudally, the scapula passing through the sternum and the spinal processes of the thoracic vertebrae, using the thoracic weighing tape. After weighing, with the Samsung cell phone camera, model A5, the animals were photographed at an average distance of 5 meters. With the aid of the ImageJ program, body area, body length taken laterally between the ventral end of the shoulder and the tip of the ischium and the height of the back measured at the backline to the curve of the ridge behind the front leg were measured. The application was developed in the Python 3.7 language, using framework Kivy. Keras frameworks with Tensorflow were used to create the bovine identification model and the Mask R-CNN for image segmentation. The data were submitted to the Shapiro-Wilk normality test, Pearson's correlation test and multiple regression analysis with the method stepwise for detecting the best predictive model through the Akaike Information Index. Statistical analyzes were performed using the R statistical program and the level of significance adopted was 5%. The predictive model selected by the statistical analysis was the one that included only the body area as a predictor variable. It presented a correlation of 0.83 (p <0.01). In this sense, it is suggested to use this predictive equation: Body Weight = 293.2 + 0.27 * Body Area (r2 = 0.68; p <0.01). The estimate of the body weight of beef cattle by body area measured from the photographic image proved to be viable.
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19
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JANAÍNA COLECHA ROCHA
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Effect of the Quality Management System on the quantitative and qualitative performance of swine insemination plants.
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Leader : IVAN BIANCHI
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MEMBRES DE LA BANQUE :
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ANA PAULA GONÇALVES MELLAGI
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ANDRÉ FURUGEN CESAR DE ANDRADE
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IVAN BIANCHI
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RAQUEL AUSEJO MARCOS
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THOMAZ LUCIA JÚNIOR
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Data: 7 août 2020
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Afficher le Résumé
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The objective of this work was to evaluate the processes of collection and manipulation of ejaculate in swine GTC with potential impact on sperm and microbiological quality of ID. Eleven boar studs distributed in the states of RS, SC, PR, MS and MT were evaluated, totaling about 1,650 boars in the total squad (around 25% of boars in the Brazilian squad in production). All units had the same computer semen analysis system (CASA; Magavision®Magapor®, Spain) in use for more than 12 months, calibrated to the supplier's standard. Quantitative and qualitative data from the semen production of the units were evaluated, based on two years of production, from August 2017 to July 2019, together with the sample evaluation of microbiology exams of the main critical points of production , DI sperm concentration and verification of the semen production process through a Check List with 75 evaluated items. The non-conforming items in the Check List of 96 visits were correlated in the period of 30 days before and after application, with the non-conforming results of the analyzes. In the evaluation of the contamination variables of pure semen, of the diluted and stored doses and in the water samples (input, post-treatment, stored and diluent), factors were found that showed significance (P<0.05), increasing the chance of damage occurring on the quality of the dose. In the concentration analysis, only the percentage of samples with a concentration >3.48 billion sperm in traditional doses was affected, with few non-standard samples for this variable. We identify the points in the process that negatively impact the quality of the dose and need to be considered according to the degree of risk.
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20
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VAMIRÉ LUIZ SENS JÚNIOR
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Influence of different feeding forms and different mixing ages on group housing system for sows on reproductive parameters and animal welfare.
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Leader : IVAN BIANCHI
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MEMBRES DE LA BANQUE :
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AMANDA PIMENTA SIQUEIRA
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THOMAZ LUCIA JÚNIOR
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JULIA EUMIRA GOMES NEVES PERINI
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IVAN BIANCHI
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Data: 7 août 2020
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Afficher le Résumé
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The objective of this experiment is to evaluate the reproductive parameters and Animal Welfare of swine females submitted to different group housing systems and differnt feeding models during gestation. The experiment was carried out in a sows unitfarm located in southern Brazil. A total of 1.080 females distributed in a 2 x 2 factorial scheme were used, with 10 repetitions over time. The factorial model consisted of two different transfer times to the group housing barns (immediately after the insemination protocol or at 35 days of gestation) and two different feeding process (“Y” or minibox drops). During the repetitions the females were equally distributed among the treatments in order of parturition (40 cycle 1 females (P1); 16 cycle 5 females and 16 cycle 6 females (P5&6) and 36 gilts). Artificial inseminations (AI) were performed with 45 ml intrauterine doses at a concentration of 1,25 billion viable sperm. From pregnancy to delivery, reproductive parameters were evaluated: return to estrus, pregnancy checked at 28 d with ultrasound, total births and average litter weight. The analysis of Animal Welfare during pregnancy was based on the Welfare Quality® protocol, evaluating the behaviour and health of females at specific moments during gestation. Behaviour data was collected 16 different times (days 1, 8, 15, 22, 29, 36, 42, 49, 56, 63, 70, 77, 84, 91, 99 and 106 after AI). Health assessment was performed on all females on days 5, 31, 40 and 101 after AI. The behavior during feeding was evaluated in gilts and primiparous animals weekly during pregnancy. There was no difference in the birth rate and in the total number of births when comparing the method of accommodation, system and interaction (P> 0.05) or for gilts, OP1 and OP5 and 6). In the evaluation of the behavior of gilts and primiparous animals during feeding, no difference was found (P> 0.05) for agonistic and active behavior when comparing system, gestation week and interaction (P> 0.05) nor for gilts, OP1 and OP5 and 6). The behavior of the females was not influenced by the system-housing interaction (P> 0.05) in any of the categories. Swine females can be handled in small collective groups (9) without minibox division. The management of the formation of collective pens can occur immediately after coverage or at 35 days.
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21
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GABRIELLA CARVALHO MATTOS FERREIRA
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Bovine babesiosis and anaplasmosis in Latin America: a systematic review and metanalysis.
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Leader : CARLOS EDUARDO NOGUEIRA MARTINS
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MEMBRES DE LA BANQUE :
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ANTONIO PEREIRA DE SOUZA
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CARLOS EDUARDO NOGUEIRA MARTINS
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DEBORA CRISTINA OLSSON
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Data: 7 août 2020
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Afficher le Résumé
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Babesiosis and bovine anaplasmosis have huge impact on cattle production (meat and milk) in Brazil, Argentina, Uruguay, Paraguay and Colombia, the producers in Latin America, despite their differences in climate, herd and management because several outbreaks are still reported. The objective of this study is to estimate the prevalence of babesiosis and anaplasmosis in the cattle of Latin America through a systematic literature review and meta-analysis of observational studies, epidemiologically characterizing these diseases and identify the relationship between climate, herd, age, sex on prevalence data. A quantitative systematic review of the literature was done, using keywords that identifiy population (“cattle OR” bovine OR “dairy” OR “dairy” or its variants), intervention (“babesi *” OR “anaplasm*” AND “ tick ”and the countries evaluated) and results (“prevalence ”OR“ epidemiolog * OR “risk factor”) on five different databases (Science Direct, Scopus, Scielo, PubMed and ISI Web of Science) using EndNote Web. It was retrieved 176 records, published between 1973 to 2019, which were subjected to peer review. Thus, 100 of which were selected for full text reading. After, 33 investigations were used in metananalysis. For Babesia spp., 17 and 6 articles were found on Brazil and Latin America, respectively. For Anaplasma marginale, 11 and 5, respectively. A substantial heterogeneity was expected and pooled prevalence was estimated by the DerSimonian and Laird random effects model and analysed comparing Brazilian’s and Latin America’s prevalence.
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22
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MARCOS CÉSAR NOUALS
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Performance evaluation of Nile tilapia fingerlings submitted to different types of food (food storage and delivery times) during the circadian cycle.
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Leader : ADOLFO JATOBA MEDEIROS BEZERRA
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MEMBRES DE LA BANQUE :
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GABRIEL FERNANDES ALVES JESUS
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ADOLFO JATOBA MEDEIROS BEZERRA
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MAURICIO LEHMANN
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Data: 10 août 2020
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Afficher le Résumé
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This work evaluated the zootechnical performance, nutrient retention and body indexes in Nile tilapia (Oreochromis niloticus), submitted to different feeding managements (times and amounts of food provided). The experiment was carried out at the Center for Aquaculture and Fishing Development (CEDAP) at the Itajaí Fish Farming Unit, located at the Itajaí Experimental Station, lasting 40 days, in 2019. 360 Nile tilapia fingerlings were used. , with an average initial weight of 23.38 ± 0.24 g, distributed in 18 experimental 1.0 m³ (1.0 mx 1.0 mx 1.0 m) volumetric capacity net cages, installed in a 300-foot pond m² with aerator. The design was a two-factorial (2 times vs 3 ways of offering) randomized blocks with three replications, totaling 6 treatments. All treatments received the same daily amount of diet, changing only the time and feeding rates at each meal. Treatments are: T1 = fish fed at 08:00, 11:00, 14:00 and 17:00 hours, with feed rates equal to 25%; T2 = fish fed at 08:00, 11:00, 14:00 and 17:00 hours, with feed rates of 10, 20, 30 and 40%; T3 = fish fed at times 08:00, 11:00, 14:00 and 17:00 hours, with feed rates 10, 40, 40 and 10%; T4 = fish fed at 11:00, 14:00, 17:00 and 20:00 hours, with feed rates equal to 25%; T5 = fish fed at 11:00, 14:00, 17:00 and 20:00 hours, with increasing portions of 10, 20, 30 and 40%; T6 = treatment 6, fish fed at 11:00, 14:00, 17:00 and 20:00 hours, with feed rates of 10, 40, 40 and 10%. Dissolved oxygen and water temperature were monitored daily. Ammonia, nitrite, pH, transparency, alkalinity and hardness, as well as biometrics to monitor the growth of animals and adjustment of the amount of feed were performed weekly. Data were subjected to Levene analysis to verify homoscedasticity. Subsequently, they were submitted to two-factor analysis (2X3). All analyzes were performed with a significance level of 5%. After 40 days, the fish remained fasting for 24 hours and all were weighed and measured to assess final weight., daily weight gain, feed efficiency, survival and productivity, which averaged 127.25 ± 4.46 g, 2, 60 ± 011 g.day -1, 1.05 ± 0.03, 99.72 ± 0.48% and 2537.93 ± 99.73 gm-³, respectively. In addition, four fish per experimental unit (72 in total) were anesthetized with clove oil (50 mg.L-1) until cercular movements ceased for later brain euthanasia and visceral fat index assessment of 1.96 ± 0. , 19% and sample collection for analysis of nitrogen retention and excretion, in the values of 54.15 ± 2.90% and 24.66 ± 2.17 kg.ton of fish-1, respectively. There was no significant difference between treatments for any of the factors analyzed. It was concluded that under the experimental conditions evaluated the time and the different distributions of the amount of food per food does not interfere with the productive and body indices, as well as the nitrogen retention capacity of tilapia juveniles subjected to the same water quality parameters.
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23
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RAFAEL SALES PAGANI
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INFLUENCE OF PUBLIC VISITATION ON THE WELL-BEING OF ANGOLAN LIONS (Panthera leo bleyenberghi), BY EVALUATING FECAL METABOLITES OF GLUCOCORTICOIDS AND BEHAVIORAL VARIABLES.
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Leader : ANA CAROLINA GONCALVES DOS REIS
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MEMBRES DE LA BANQUE :
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ANA CAROLINA GONCALVES DOS REIS
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ELIZABETH SCHWEGLER
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NEI MOREIRA
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Data: 4 sept. 2020
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Afficher le Résumé
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The natural lion populations have been declining in recent decades due to anthropic pressure, requiring population management under human care. Such actions are only acceptable given aspects of animal welfare. The objective of this study was to evaluate the effect of the number of visitors at the zoo on the welfare of Angolan lions, through individual behavioral analyzes and fecal glucocorticoid metabolites (MFG). Pomerode Zoo houses four male Angolan lion, objects of the study, aged between five and six years. The collections were carried out between 06/06/2019 to 07/07/2019. Fecal samples were collected individually in the morning and deferred by ingesting seeds. They were stored at -20 ° C until sent to the Laboratory of Hormonal Dosages of the Faculty of Veterinary Medicine of the University of São Paulo and processed by enzyme immunoassay. The validation of the exams was obtained through a challenge with adrenocorticotropic hormone. Etograms were performed by scanning method, every 1 minute, for two hours in the morning and in the afternoon. The number of visitors was obtained from the administrative sector and the meteorological data was obtained from the National Institute of Meteorology. The statistical analysis was performed using the SAS. The number of visitors and weather conditions did not influence the MFG. There was also individual variation, where one of the lions was more likely to have higher MFG values. Regarding behavioral analysis, when the number of visitors was higher, the animals remained more in places with sand and in the quadrant closest to the visitors. With the results it is concluded that these individuals of Angolan lions are not negatively influenced by the increase of visitors in the zoo, because they have dynamic environment and protocols of medicine, nutrition and welfare that attend the needs of the species.
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24
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KELEN REGINA ASCOLI BALDI
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Comparison between direct immunofluorescence and immunohistochemistry for the diagnosis of Listeria monocytogenes in formalin-fixed paraffin-embedded tissues.
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Leader : TEANE MILAGRES AUGUSTO GOMES
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MEMBRES DE LA BANQUE :
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DIOGENES DEZEN
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JOÃO XAVIER DE OLIVEIRA FILHO
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MARCIA CRISTINA DA SILVA
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TEANE MILAGRES AUGUSTO GOMES
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Data: 23 oct. 2020
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Afficher le Résumé
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Listeria monocytogenes is a bacterium that infect humans and animals and causes a zoonotic disease characterized by encephalitis, septicemia or abortion. Also, listeriosis leads to significant economic losses due to animal death or sacrifice. The aim of this work was to compare the technique of direct immunofluorescence (IFD) and immunohistochemistry (IHC) for the diagnosis of L. monocytogenes in formalin-fixed and paraffin-embedded (FFPE) tissues. A total of 25 tissue blocks from animals with history and/or lesions compatible with listeriosis were selected. For immunohistochemistry, a diluted polyclonal anti-L. monocytogenes serotype 1 and 4 primary antibody was used, followed by a secondary antibody with polymer kit. For immunofluorescence, the same primary antibody wasused, followed by fluorescein-labeled anti-rabbit IgG secondary antibody. Each samplewas classified according to the immunostaining presence and intensity. From 25 samples, 10 were positive at least for one technique, whereas eight samples were positive for both IHC and IFD with similar intensity. There was strong immunolabeling in samples from bovines experimentally infected with L. monocytogenes ATCC 7644, as well as in nervous tissues from naturally infected ruminants. This study demonstrated that both techniques are efficient to detect L. monocytogenes in FFPE tissues. Using processed biological materials for IFD, instead of fresh samples, is a quite unique technique, since there are few protocols described. However, IFD did not show any difference in sensitivity when compared to IHC, whereas the latter is less expensive and has an easier result interpretation.
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25
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MAELY PETRY
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Physico-chemical, sensory and microbiological profile of Blue Shark (Prionace glauca) eviscerated on board.
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Leader : ROBILSON ANTONIO WEBER
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MEMBRES DE LA BANQUE :
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ROBILSON ANTONIO WEBER
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ADOLFO JATOBA MEDEIROS BEZERRA
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GILBERTO CAETANO MANZONI
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Data: 11 déc. 2020
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Afficher le Résumé
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The quality of the fish is directly related to the handling of the fish in the capture vessels and the time they remain stored until they are processed in the industry. The project aimed to determine the quality profile of the Prionace glauca eviscerated on board. Three treatments were applied with 16 repetitions: captured and stored for up to 5 days; captured and stored from 6 to 10 days; and captured and stored for more than 11 days. All samples collected were from the same vessel and cruise. An average of 900 g of muscle per individual was collected, and these were fractionated, stored in plastic bags, and frozen for later delivery to the laboratory. These samples were subjected to physical-chemical, microbiological and sensory analyzes, the results being compared with what is recommended by the laws that support the quality of the fish. It was observed that the pH in all groups was above allowed by the legislation. The level of mercury in all samples was significantly below the maximum allowed limit. Only one sample was positive for Salmonella spp., indicative of contamination at the time of evisceration. As for the levels of total volatile bases, the results obtained showed that this index is not a trustworthy criterion for indicating degrees of freshness. Sensory parameters were different in all groups evaluated. Based on the results we concluded that the quality of the meat of P. glauca is influenced by the storage time, by the good handling practices on board, and that the concentration of mercury in this species that inhabits brazilian waters is minimal, when compared to other catch regions in the world.
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26
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FABIANO DE LIMA MATTER
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Evaluation of Propofol as an anesthetic in Ictalurus punctatus.
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Leader : ROBILSON ANTONIO WEBER
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MEMBRES DE LA BANQUE :
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ROBILSON ANTONIO WEBER
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CARLIZE LOPES
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ÉVERTON LUÍS ZARDO
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Data: 14 déc. 2020
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Afficher le Résumé
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The present study aimed to evaluate Propofol (2,6 - diisopropylphenol) as anesthetic in Catfish (Ictalurus punctatus) and its effects on fish physiology. The project was divided into two phases. Phase I: a) Determination of the ideal anesthetic concentration. Phase II: Evaluation of the anesthetic's effect on physiological responses (cortisol, glucose, lactate) and histopathological changes in gills and liver. To determine the ideal dose, 60 juveniles with an average weight of 96.60 ± 27.70 g were used, submitted to different concentrations of anesthetic: 1.5; 2.0; 2.5; 3.0; 4.0; 5.0; 6.0 mg/L. The concentration of 2.5 mg/L was considered as the ideal concentration, providing anesthetic induction and recovery in less time. The evaluation of the anesthetic's effect on the physiological responses and histopathological changes showed that the exposure of juvenile catfish to a concentration of 2.5 mg/L of propofol was sufficient to cause changes in the values of the variables: cortisol, glucose, lactate and histology of liver and gills. However, there was no significant difference in plasma cortisol levels (P>0.05) between the control and treatment groups. The histopathological study revealed mild to moderate changes in the gills (congestion in the lamellar vessels and central vein, epithelial elevation), moderate and severe changes in the liver (congestion and degeneration). Mortality rates were not observed. This study indicates that the 2.5 mg / L dose of propofol provides safe anesthesia in I. punctatus and suppresses the increase in the level of cortisol.
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27
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LEONARDO BENASSI DE BORBA
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Antibiotics for veterinary use: Preliminary assessment of phytotoxicity and impact on the enzymatic activity of soil microorganisms.
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Leader : CLEDER ALEXANDRE SOMENSI
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MEMBRES DE LA BANQUE :
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CLEDER ALEXANDRE SOMENSI
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CRISTIANE VANESSA TAGLIARI CORREA
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RENAN CHIPRAUSKI TESTOLIN
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Data: 29 déc. 2020
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Reduction of environmental impacts is one of the main challenges in animal production, since the high population density of animals leads to increased pressure of infection in the facilities and in order to maintain sanity and increase productivity, there is a frequent use of antimicrobials. Thus, in addition to the large production of waste products (i.e., organic matter), the situation is aggravated by the presence of several biologically active drugs in these waste products, which are often used as biofertilizers. Veterinary drugs are considered emerging pollutants, with antibiotics being the most harmful class, as they are biologically active molecules that will cause deleterious effects on phytoproductivity and soil microbiology and predispose to the appearance of superbacterias due to the effect of selective pressure. Soil enzymatic activities are often used to monitor impacts of management, agricultural practices or environmental contamination. One of the techniques used to quantify enzymatic activities is the use of fluorescein diacetate (DAF) hydrolysis, suggested as a possible indicator to measure the global microbial activity of a soil. Therefore, the objective of this work was to evaluate the impact of veterinary antibiotics on phytoproductivity and soil microbiology through the hydrolysis of fluorescein diacetate. The soil was collected in the city of Camboriú - SC and was submitted to hydrolysis analysis of fluorescein diacetate and to the phytotoxicity test with the species Lactuca sativa (lettuce). According to the results, the most appropriate exposure time to assess the impact of antibiotics on soil microbiology was 24 hours, while the incubation time for the analysis of the hydrolysis of DAF that demonstrated the best results was 3 hours. In general, both Amoxicillin and Norfloxacin showed less DAF hydrolysis activity in the soil compared to the control soil. For the dry and wet biomass parameters of lettuce, colistin 2% and ampicilin 1% were the treatments that demonstrated the greatest negative influence on the seedling biomass of lettuce, demonstrating the phytotoxic potential of antibiotics. In this way, the work makes it evident that the use of excessive antibiotics in animal production can lead to environmental impacts and, in the future, to public health problems.
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